Abstract: In order to investigate the epidemiology of extended-spectrumβ-lactamases-producing E.coli and to detect CTX- M type ESBLs in Henan province,the E.coli strains isolated from chickens and pigs were screened for the presence of ESBLs by phenotype confirmatory test,and theCTX- M type ESBLs were detected by PCR. The results indicated the positive rates of ESBLs among E. coli stains isolated from chickens and pigs were 62. 8 %(59/ 94) and 78. 3 %(36/ 46),respectively.Further PCR amplification results revealed that the positive rate of the CTX- Mtype ESBLs in chickens was 89. 8 %(53/ 59).Of them,there were 14 isolates positive for CTX-M-2 group,26 isolates for CTX- M- 9 group,13 isolates for both CTX-M-2 and CTX-M-9 group.While,the positive rate of the CTX-M type ESBLs in pigs was 69.44 %(25/ 36).Of them,there were seven isolates positive for CTX- M- 2 group,17 isolates for CTX- M- 9 group,one isolate for both CTX-M-2 and CTX-M-9 group.The positive rate of CTX-M type ESBLs-producing Escherichia coli strains is relatively high in Henan province,with the CTX-M-2 group and CTX-M-9 group dominant.

Key words: Escherichia coli, Resistance, CTX-M

CLC Number: 

• S32