Determining the Glycocholeic Acid of Rats Blood Serum with SPE HPI, C

doi:10.7668/hbnxb.2009.01.048

Abstract

Abstract: In this paper,determination method of concentrations of glycocholeic acid in rats blood serum is estab-lished by reversed phase high perfoimance liquid chmmatography(RP-HPLC),This study aims to understand the varia-dons of concentrations of glycocholeic acid in rats blood serum,provide a new method for Clinical Detection of gycochole-is acid,development useful drug from alnimal source. Uses the HPLC law,the chromatographic column is Waters XterraRP18 (150 mm X4. 6 mm,5μm),flowing for second grade nitrile- KH₂PO₄solution,examination wave length 213 nm,col-umn warm 30 ℃,specimen handling plume l0 L. The result the glycocholeic acid density and the peak area assumes thegood linear relations in 0. 156 25-10. 0 μg/ mL scope,regression equation Y=39 579 X+57 823,R²=0. 998 4; Takesignal-to-noise ratio S/ N=3 as the standard,the lowest examination density is 0. 02μg/mL, The average recovery was98. 49%;the RSD of innerday was 0. 52%-2. 00%,the RSD of infra day was 0. 74%-4. 98%.The method was sinrple,accurate,sensitive,feasible and suitable for analyzing glycocholeic acid concentration in blood serum.

Key words: SPE, HPLC, Blood serum, Glycocholeic acid

CLC Number:

R285

LI Shao-chun, LI Pei-feng. Determining the Glycocholeic Acid of Rats Blood Serum with SPE HPI, C[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 215-218. doi: 10.7668/hbnxb.2009.01.048.

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