Effects of Egg Yolk and LDL on Changes of Cholesterol and Phospholipids in Sheep Spermatozoa during Freezing

doi:10.7668/hbnxb.2008.06.026

Abstract

Abstract: For search the reasons of low pregnant rate of sheep frozen semen,the paper studied on the changes of cholesterol and phospholipids in sheep spermatozoa during freezing,which was protected with egg yolk or low density lipoprotein(LDL)in diluents.The results indicated that the contents of cholesterol of sheep spermatozoa decreased from 234.54 nmol/10⁹sperm of fresh semen to 163.76 nmol/10⁹sperm of frozen semen and the ratio of cholesterol and phos-pholipids(c/p)decreased also from 0.54 to 0.36 when egg yolk was added in diluents.When egg yolk in diluents was instead with LDL 9 g/100mL,content of cholesterol in spermatozoa was 243.73 nmol/10⁹sperm after frozen and c/p was also maintained 0.50.In conclusion,the content of cholesterol and c/p in sheep spermatozoa decreased during freezing in egg yolk extender and LDL could prevent the content of cholesterol and c/p from decreases.

Key words: Sheep, Semen frozen, Egg yolk, Low density lipoprotein(LDL), Phospholipids, Cholesterol, Ratio of cholesterol and phospholipids(c/p)

CLC Number:

S826

ZHENG Yun-sheng, HONG Wei, NI Li-ping, TIAN Dong-hai, LU Yong-yue, DENG Chang-sheng, LI Jin-quan. Effects of Egg Yolk and LDL on Changes of Cholesterol and Phospholipids in Sheep Spermatozoa during Freezing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 112-115. doi: 10.7668/hbnxb.2008.06.026.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.06.026

http://www.hbnxb.net/EN/Y2008/V23/I6/112

[1]	YANG Yawen, GAO Hexuan, LIU Lili, BAO Yingying, HE Yuqin, YANG Zhijie, CHEN Weigang, GE Wenbo. *Dynamic Changes of Plasma Kisspeptin and Expression and Distribution of Kiss1/GPR54* in PHPT Axis of Ganjia Tibetan Sheep During Estrus Cycle** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 204-212.
[2]	LIANG Peng, ZHANG Wen, FENG Dengzhen, QIANG Hao, RONG Xuan, MENG Ke. Screening Candidate Genes Related to Meat Quality Traits in Sheep Based on Transcriptome [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 220-231.
[3]	MA Xiaojun, WANG Jiamian, LI Shiying, YAN Lijiao, MA Qiangsheng, ZHANG Xiaoli. *Effects of E.coli* Infection on Fibroblast Injury and TLR4 Expression in Sheep Mammary Glands** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 231-238.
[4]	SHI Fengyun, ZHU Guangqin, LIAO Yunqiong, WANG Bing. *Correlation Analysis of IGFBP-3* Gene Nucleotide Variation and Ovine Slaughter Traits** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 368-372.
[5]	CUI Baowei, LIU Quanyong, QIN Guangli, ZHU Junpeng. Effect of Combined Application of Fermented Sheep Manure and Soil Loosening and Root Promoting Agent on Soil Physical and Chemical Properties Yield and Quality of Red Cluster Pepper [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 182-187.
[6]	RONG Heng, ZHANG Mengyao, HE Jianning, LIU Nan. *Study on Construction of BMP6* and BMPR1B Expression Vectors by Cognate Recombination and Co-transfection of Fibroblasts** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 195-201.
[7]	ZHANG Mengyao, YANG Feng, LIU Kaidong, LIU Jifeng, LIU Nan, HE Jianning, XUE Ming. BMP4 Gene Plasmid Construction and Its Expression Amount in Fibroblasts of AoHan Fine Wool Sheep [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 227-234.
[8]	WANG Xiaolong, LIU Fengzhi, SHI Xiangbin, WANG Xiaodi, JI Xiaohao, WANG Zhiqiang, WANG Baoliang, ZHENG Xiaocui, WANG Haibo. Effects of Organic Fertilizers on Root Growth and Soil Nutrition of Grape [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 177-184.
[9]	ZHANG Mengyao, YANG Feng, LIU Jifeng, LIU Kaidong, HE Jianning, LIU Nan. *Study of Aohan Fine Wool Sheep Hoxa5* Gene and BMPR1B Gene Interaction** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 229-238.
[10]	LIU Xiu, ZHANG Yaqiang, LI Shaobin, WANG Jiqing, HU Jiang, SHA Yuzhu, ZHANG Wei, LUO Yuzhu. *Analysis of EGLN1* Gene Expression, Mutation Characteristics and Adaptability in Tibetan Sheep** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 226-231.
[11]	HUA Liushuai, WANG Jing, BAI Jie, ZHU Xiaoting, CHEN Fuying, YU Xiang, CHU Qiuxia, XIN Xiaoling, XU Zhaoxue, ZHAO Hongchang, WANG Eryao. *Lentivirus Mediated Construction of Stably Expressing FABP4* Fibroblasts Line from the Small-tailed Han Sheep** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 121-126.
[12]	LIANG Ruiyuan, CHEN Xiaoyong, SUN Hongxin, SUN Shaohua, DUN Weitao, SHEN Zhiqiang, LIU Junfeng, WANG Jue. Phylogenety and Genetic Diversity of Five Local Sheep Breeds on mtDNA D-loop [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 135-142.
[13]	MA Xiaojun, CHEN Fuqiang, ZHANG Xiaoli, LI Fadi, TANG Ran, ZHANG Chen, SONG Xiaoyu, ZHANG Xin. *Effect of Staphyloccocus aureus* on TLR2 Expression in Mammary Glands of Small-tail Han Sheep** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 50-55.
[14]	ZHU Jianxun, WANG Jiqing, HU Jiang, LIU Xiu, LI Shaobin, YAN Wei, LUO Yuzhu. *Nucleotide Sequence Variations of KAP11-1* Gene in 5 Chinese Sheep Populations** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 101-107.
[15]	WANG Hua, WEI Yubing, TONG Jianwei, ZHANG Mingjun, WANG Jianfu, WANG Xinrong, CHENG Shuru. PRKAG3 Gene SNPs of Sheep and Correlation Analysis of Meat Quality Traits [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 134-140.

Please choose a citation manager

Content to export