Abstract: Nitrogen fertilizer effects on the grape leaves′ growth and the anatomical changes both of the leaves and roots through different levels of nitrogen fertilizer applications.The results express that the leaves′ length,width and leaf area are increased with the amount of nitrogen fertilizer applications′ increasing,which is also same to the content of chlorophyll,Especially,chlorophyll a increased sharply,while the chlorophyll b didn′t increase in evidence.Then the chlorophyll fluorescence quantum absorptivity is increased strongly,which is same with the chlorophyll a/b′s trend of change.That indicates quantum absorptivity is in relation with the chlorophyll a.Samples are observed by the cross sections,after the staining by DAPI,as the amount of nitrogen fertilizer applications increasing,nucleolus amount of the leaves′ is not changed in evidence,while the nucleolus amount in the root apiex meristem is increased sharply.After the staining by the I2-KI,also the starch content of both the leaves and roots is increased in evidence as the amount of nitrogen fertilizer applications increasing.

Key words: Grape, Nitrogen fertilizer, Leaf, Root

CLC Number: 

• S663.1