Abstract: The paper was based on traditional soil microorganism isolated culture method, it made groping research to affect bacteria, fungi and actinomycetes isolated effects several important factors selected several important influencing fae- tars respectively though the direct cross test and single factor test. The goal is to discuss and solve present situation that isolation and culture of microrganism in soil is difficult through the practical and feasible means. The experiment has conducted the separation characteristics research of bacteria, funguses and actinomyces through culture medium screening, the different diameter of glass bead hitting, as well as chemistry dispersing agent and the duration of oscillation and so on four factors orthogonal designs. At the sane time, there is two factor single factor experiments including the heat treatment temperature of soil sample and the time of heat treatment on isolation of act dinomyces. The result of Researching indicated that bacteria's、separating optimal combination is SABA PYJ+ Big beading(Ф=5-7 mm, 55 grains)+ no Sodium De-oxycholate+ Vibrating time 90 min; Fungi's、separating optimal combination is MA PYj+ Big bead in+ 1% Sodium Deoxy-cholat Vibratirg time 60 min; Actinomycetes' separating optimal combination is SGA PYJ+ 25℃ treatment+ Big beading+1% Na4P2O710H20+ Vibrating time 60 min. The variational law of some factors, single and repeat tests were similar with the Direct Cross Test result.

Key words: Soil, Microorganism separaation, Culture medium, Direct cross test

CLC Number: 

• S714.5