Abstract: Rooting and hardening-off culture were specially performed to solve some problems emerged in previous embryo rescue of Chinese persimmon cv. Mopanshi. The contrast designs showed that adding 1% Chinese ink in the basic medium for germination could raise the percentage of plantlet formation by 87%. In hardening-off culture stage, cutting the main root and sucrose-free culture could improve the development of lateral roots and the nutrient acclimation of hybrid plantlets. By combining use of above three measures, more than 96% embryos were successfully rescued and transplanted to field.

Key words: Mopanshi persimmon, Embryo rescue, Rooting and hardening-off culture

CLC Number: 

• S665.2