The Establishment of a New Genetic Transformation System by Agrobacterium tumefaciens-mediated in Germinating Embryo of Maize

doi:10.7668/hbnxb.2007.05.027

Abstract

Abstract: A new genetic transformation system by Agrobacterium tumefaciens-mediated in germinating embryo of maize was established. The result showed that integration of foreign gene into the genome of transgenic maize plants was confirmed by PCR, PCR-Southern and RT-PCR analysis. The foreign gene was expressed at transcription level. According to this method, the procedure of plant tissue culture was avoided and the germinating embryo of maize was obtained conveniently. It will provide an efficient way for genetic transformation of maize.

Key words: Agrobacterium tumefaciens, Maize, Germinating embryo, Genetic transformation

CLC Number:

S513.01

WANG Chang-tao, ZHAO Yu-jin, LI Kun-yuan, ZHANG Shi-huang. The Establishment of a New Genetic Transformation System by Agrobacterium tumefaciens-mediated in Germinating Embryo of Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(5): 110-113. doi: 10.7668/hbnxb.2007.05.027.

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http://www.hbnxb.net/EN/Y2007/V22/I5/110

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