The Association between the Polymorphism of ATG16L1 and Non- specific Digestive Disorder in Rabbit

doi:10.3969/j.issn.1000-7091.2013.04.043

Abstract

Abstract: To study the susceptibility of ATG16L1 gene to non- specific digestive disorder (NSDD) in rabbit， single nucleotide polymorphism (SNP) of rabbit ATG16L1 gene was firstly detected by PCR- HRM and the associa- tion analysis of ATG16L1 gene mRNA expression in colon of fibre- deficient diet group was conducted．The results found that there was a SNP locus (c． 1482 G ＞ A) in ATG16L1 gene and the SNP locus was associated with the sus- ceptibility to NSDD; the allele A of c． 1482 G ＞ A could increase the susceptibility (OR = 1． 43， 95% confidence interval: 1． 08－ 1． 91， P ＜ 0． 05); in recessive genetic model，the genotype AA could increase the susceptibility (OR = 1． 69，95% confidence interval: 1．05－ 2．78，P ＜ 0．05); along with the increasing severity of non- specific digestive disorder， the ATG16L1 gene mRNA expression in colon was gradually increased (P ＜ 0．05); genotype AA had the lowest mRNA expression in fibre- deficient diet group (P ＜ 0．05) ．These results showed that ATG16L1 gene was associated with the genetic susceptibility to NSDD in rabbit．

Key words: Non- specific digestive disorder, ATG16L1, SNP, Rabbit

CLC Number:

S829.1

XIAO Zheng-long, ZHANG Gong-wei, YANG Yu, WANG Jie, LAI Song-jia. The Association between the Polymorphism of ATG16L1 and Non- specific Digestive Disorder in Rabbit[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 234-238. doi: 10.3969/j.issn.1000-7091.2013.04.043.

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