Effects of the Protein Toxin from Xenorhabdus nematophila on Several Enzymatic Activities of the Helicoverpa armigera Larvae

doi:10.3321/j.issn:1000-7091.2007.03.025

Abstract

Abstract: ToxinⅡ, isolated from the cell of X. nematophila HB310, was an oral insecticidal protein complexes. The effects of the toxinⅡon the growth disrupting and several enzymatic activities of the Helicoverpa armigera larvae were investigated. The results indicated that the larvae period from 4th instar to pupae was 6.65 d, it remitted 2 d as compared with that of control. And the pupation rate was inhibited by toxinⅡ, the pupation rate of the treated reached 62%. The average weight of pupae was 106.47 mg, it was significantly less than that of control. The activities of weak alkaline trypsin-like enzyme, active alkaline trypsin-like enzyme, chymotrypsin-like enzyme and total protease in the toxin-treated midgut were inhibited significantly at 36 h, which were 0.3708, 0.1903, 0.3282 and 0.2141 times of those of the control respectively. On the other hand, the activities of carboxylesterase, esterase and acetylcholinesterase in the toxintreated midgut were peaks at 36 h, which were 15.66, 9.49 and 6.38 times than those of the control respectively.

Key words: Xenorhabdus nematophila, Helicoverpa armigera, Protease, Detoxicating enzyme, Acetylcholinesterase

CLC Number:

S482

SHI Cui-hong, SONG Ping, WANG Qin-ying, YANG Jun, CUI Long, KONG Fan-fang. Effects of the Protein Toxin from Xenorhabdus nematophila on Several Enzymatic Activities of the Helicoverpa armigera Larvae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(3): 105-110. doi: 10.3321/j.issn:1000-7091.2007.03.025.

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