Molecular Identification of Potato virus S Hebei Isolate

doi:10.3321/j.issn:1000-7091.2007.03.010

Abstract

Abstract: A isolate of Potato virus S (PVS-Hebei) was obtained from potato plants cultivated in Zhangjiakou of Hebei province and the coat protein (CP) gene was colned and sequenced. The CP gene was amplified from total extracted potato RNA by reverese transcription-polymerase chain reaction (RT-PCR). The specific PCR fragment then cloned into the pMD18-T vector and sequenced. The result showed that the cloned segment was 714 bp and encodes 294 amino acid residues with relative molecular weight of 33 kD which is the length of the PVS CP, Comparison of the nucleotide sequence between the CP gene and those of several PVS isolates showed that the homology of are 82.4%-95.6%, and the homology of the putative amino acid sequence are 93.9%-98.0%, respectively. The evolutionary distances analysis suggested that PVS isolates was divided into two groups, PVS-Hebei was assigned into groupⅠand that it was close to the ordinary European isolates of ordinary strain of PVS (PVS-Uk) but distant to the original Andean strain of PVS (PVS-A).

Key words: Potato virus S, Coat protein gene(CP), Sequence analysis, Isolate identification

CLC Number:

S436.32

ZHOU Qiao-mei, XIE Xiao-liang, WEN Chun-xiu, MA Hui, YIN Jiang, WU Zhi-ming. Molecular Identification of Potato virus S Hebei Isolate[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(3): 39-42. doi: 10.3321/j.issn:1000-7091.2007.03.010.

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