Indirect ELISA for Detection of Verticillium dahliae and Its Application

doi:10.3321/j.issn:1000-7091.2007.02.035

Abstract

Abstract: Mycelium protein of Verticillium dahliae was used for the production of polyclonal antibodys to V. dahliae, and a indirect ELISA method for specific detection of cotton verticillium wilt was established. The Mycelium protein of V. dahliae could be detected as low as 5 ng/mL at 405 nm. V. dahliae could be quantitively detected in the stem, root, leaf and soil from the diseased cotton plants through this method. Results also showed that amounts of the pathogen from the stems, roots and the soil of the diseased plants were positive correlation to the severity of cotton verticillium wilt. It convinced that the new established Elisa method is a promising technique for early detection and forecast of cotton verticillium wilt.

Key words: Cotton verticillium wilt, Mycelium protein, Polyclonal antibody, ELISA, Detection

CLC Number:

S435.62

LIU Cui-fang, GAO Guang-rui, TIAN Shi-min, JIANG Ji-zhi, LU Xiu-yun, MA Ping, LI She-zeng. Indirect ELISA for Detection of Verticillium dahliae and Its Application[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 145-148. doi: 10.3321/j.issn:1000-7091.2007.02.035.

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