cDNA Fragment Clone and Sequence Analysis of Cadherin-like Protein Gene in Tobacco Budworm (Helicoverpa assulta Guenée)

doi:10.3321/j.issn:1000-7091.2007.01.036

Abstract

Abstract: Cadherin-like protein acts as a receptor of Bt toxin by binding with toxin in brush border membrane of insect. Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene. cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid. The sequence analysis showed that 1335 bp nucleotide fragment that encodes 444 amino acid residues was located in the encoding frame. Compared with cadherin-like protein of Helicoverpa armigera and Heliothis virescens, the identity of amino acid sequence were 95% and 86%, respectively.

Key words: Helicoverpa assulta Guené, e, Cadherin-like protein, Gene cloning, Sequence analysis

CLC Number:

S433.4

LI Wei-hua, MIAO Xue-xia, HUANG Yong-ping, AN Shi-heng, GUO Xian-ru. cDNA Fragment Clone and Sequence Analysis of Cadherin-like Protein Gene in Tobacco Budworm (Helicoverpa assulta Guenée)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 152-155. doi: 10.3321/j.issn:1000-7091.2007.01.036.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2007.01.036

http://www.hbnxb.net/EN/Y2007/V22/I1/152

References

[1] McGaughey W H. Insect resistance to the biological insecticide Bacillus thuringiensis[J]. Science, 1985, 229 (4709): 193-195.
[2] Gunning R V, Dang H T, Kemp F C, et al. New resistance mechanism in Helicoverpa armigera threatens transgenic crops expressing Bacillus thuringiensis CrylAc toxin[J]. Appl Environ Microbiol, 2005, 71(5): 2558-2563.
[3] Francis B R, Bulla L A Jr. Further characterization of BTR1, the cadherin-like receptor for Cry1Ab toxin in tobacco hornworm (Manduca sexta) midguts[J]. Insect Biochem Mol Biol, 1997, 27(6): 541-550.
[4] Gahan L J, Gould F, Heckel D G. Identification of a gene associated with Bt resistance Heliothis virescenss[J]. Science, 2001, 293(5531): 857-860.
[5] Xu X, Yu L, Wu Y. Disruption of a cadherin gene associated with resistance to Cry1Ac delta-endotoxin of Bacillus thuringiensis in Helicoverpa armigera[J]. Appl Environ Microbiol, 2005, 71 (2): 948-954.
[6] Hua G, Jurat-Fuentes J L, Adang M J. Bt-R1a extracellular cadherin repeat 12 mediates Bacillus thuringiensis Cry1Ab binding and cytotoxicity[J]. J Biol Chem, 2004, 279 (27): 28051-28056.
[7] Dorsch J A, Candas M, Griko N B, et al. Cry1A toxins of Bacillus thuringiensis bind specifically to a region adjacent to the membrane-proximal extracellular domain of BT-R (1) in Mandluca sexta: involvement of a cadherin in the entomopathogenicity of Bacillus thuringiensis[J]. Insect Biochem Mol Biol, 2002, 32 (9): 1025-1036.
[8] Nagamatsu Y, Toda S, Koike T, et al. Cloning, sequencing and expression of the Bombyx mori receptor for Bacillus thuringiensis insecticidal CryIA (a) toxin[J]. Biosci Biotechnol Biochem, 1998, 62 (4): 727-734.
[9] Vadlamudi R K, Weber E, Ji I, et al. Cloning and expression of a receptor for an insecticidal toxin of Bacillus thuringiensis[J]. J Biol Chem, 1995, 270 (10): 5490-5494.
[10] 王桂荣, 吴孔明, 梁革梅, 等. 棉铃虫中肠钙粘蛋白基因的克隆、表达及Cry1A结合区定位[J]. 中国科学C辑, 2004, 34(6): 537-546.
[11] Morin S, Biggs R W, Sisterson M S, et al. Three cadherin alleles associated with resistance to Bacillus thuringiensis in pink bollworm[J]. PNAS, 2003, 100(9): 5004-5009.
[12] Xie R, Zhuang M, Ross L S, et al. Single amino acid mutations in the cadherin receptor from Heliothis virescenss affeet its toxin binding ability to Cry1 A toxins[J]. J Biol Chem, 2005, 280(9): 8416-8425.
[13] 安世恒, 郭线茹, 罗梅浩, 等. 烟夜蛾幼虫中肠CrylAc毒索受体蛋白cDNA片断的克隆和序列测定[J]. 华北农学报, 2005, 20(2): 84-88.
[14] Coates B S, Sumerford D V, Hellmich R L, et al. Sequence variation in the cadherin gene of Ostrinia nubilalis: a tool for field monitoring[J]. Insect Biochem Mol Biol, 2005, 35 (2): 129-139.

[1]	ZHAO Jie, LIU Hongquan, ZHAO Yun, YANG Kai, FU Yongbin, GU Yuzhang, SUN Lijing, HU Mengyun, LI Hui, ZHANG Yingjun. Composition and Distribution of Wheat VRN Genes and Their Relationship with Vernalization in the Northern China Winter Wheat Region [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 62-67.
[2]	ZHOU Xinqiao, CHEN Dagang, SHAN Zelin, GUO Jie, CHEN Pingli, CHEN Ke, LIU Chuanguang, CHEN Youding. Study on Dynamic Plant Type Characteristics of Different Double Cropping Super Rice in South China [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 68-77.
[3]	LI Haodong, QIN Mengfan, ZHANG Miao, WANG Rongrong, XU Yu, SONG Jia, ZHU Yunlin, HUANG Zhen, XU Aixia. *Effects of Leaf Physiology and Pistil/Stamen Development of Brassica napus* L.to Low Temperature During the Flowering Stage** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 78-86.
[4]	XIAO Jibing, LIU Zhi, XIN Zongxu, CHEN Guoqiu, WU Hongsheng. Drought Tolerance Identification of Foxtail Millet Germplasm Resources Based on GGE Biplot [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 87-96.
[5]	JIN Yifeng, GAO Yansong, WANG Qi, WANG Mengmeng, ZHAO Di, XIONG Yi, CHEN Yang. *Cloning of SnRK2.4* Gene and It's Response to Abiotic Stress in Kentucky Bluegrass** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 9-15.
[6]	ZHAI Lichao, ZHANG Lihua, ZHENG Mengjing, LÜ Lihua, SHEN Haiping, YAO Haipo, JIA Xiuling. Responses of Kernel Position Effect of Summer Maize to Increasing Plant Density and Its Carbon and Nitrogen Metabolism Characteristic [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 97-104.
[7]	LIU Shuai, XU Xuexin, ZHAO Jinke, QU Wenkai, HAO Tianjia, MENG Fangang, JIA Jing, ZHAO Changxing. Effects of Nitrogen Application Rate and Frequency on Photosynthetic Characteristics,Protective Enzyme Activities and Yield of Summer Maize under Drip Irrigation [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 114-123.
[8]	LI Xia, LUO Lihui, ZHOU Ya, YANG Dingqing, WANG Peng, LI Sen. Effects of Straw Returning on Soil Active Organic Carbon Components and Enzyme Activities in Rice-Rape Rotation [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 124-131.
[9]	WANG Lijuan, LIU Dan, XU Yongqing, FENG Xu, HE Fumeng, LI Aiyu, WANG Xue, YANG Yan, LI Cuiting, YUAN Qiang, LI Fenglan. Analysis of Physical and Chemical Characteristics and Microbial Diversity of Corn Straw in Different Maturity Period in Cold Area [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 132-139.
[10]	CHEN Qiuyuan, ZHAO Shuaibing, LIANG He, LIU Ye, CUI Yanni, PENG Ting, ZHAO Quanzhi, ZHANG Jing. Soil Bacterial Community Characteristics and Ecological Function Prediction at Different Rice Growth Stages [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 140-149.
[11]	HAN Xiaoyu, LI Qinglun, JIANG Xinglin, WANG He, YANG Lingling, SHI Yajuan, LI Honglian, CHEN Linlin, YANG Xue, SHI Yan. Localization,Interaction and Pathogenicity of BYDV GAV Encoded Proteins [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 150-156.
[12]	WANG Ya, WANG Yuetao, SHEN Guanwang, WANG Fuhua, WANG Shengxuan, BAI Tao, YIN Haiqing. Improvement of Rice Blast Resistance by Pyramiding the R Gene Pigm and the Non-R Gene bsr-d1 [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 157-165.
[13]	QU Dong, YAN Fei, LIU Xinrui, KANG Xue, ZENG Haitao, ZHANG Yu. *Cloning and Expression Analysis of AcWRKY70* Gene Sequence under Psa and Hormone Treatment in Kiwifruit** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 166-173.
[14]	SU Fang, HAN Diangang, YE Lingling, ZHANG Chong, YIN Shanglian, LUO Qianmin, DONG Xianlan, LI Yaoyao, LI Lingfeng, AI Jun, XIN Jige. Expression of COVID-19 Virus N Gene in Insect Cells [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 174-180.
[15]	WANG Shuo, ZHAO Gangyi, SHI Tianle, WU Sifan, YAN Qianying, HAN Shengfang, WANG Dongmei. Effect of Exogenous Melatonin Injection on Resistance of Wheat to Leaf Rust [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 181-186.

Please choose a citation manager

Content to export