Abstract:
Because of high destruction and degressive quality of DNAs from deeply-processed transgenic crops products,it becomes difficult to isolate DNAs,and straightly affects the sequent qualitative detection of transgenic genes.For this problem,the DNA isolation experiments were established with the raw materials of soy sauces and tomato pulps.Directed to high level of caramel in soy sauces and low pH of tomato pulps,the procedures of sample pretreatment were added before the routine CTAB methods.The PCR detection of specific native genes of soybean and tomato were applied,the results reported that,the established methods were simple,quick,efficient and sufficient for the need of the qualitative detection of GMOs.
Key words:
Soy sauces,
Tomato pulps,
DNA isolation,
Specific native genes,
PCR amplification
CLC Number:
JIN Hong, ZHAO Xin, WANG Yong, CHENG Yi. A DNA Isolation Method for the Qualitative Detection of Transgenic Deeply-processed Products[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(2): 53-56. doi: 10.3321/j.issn:1000-7091.2006.02.013.