Study on Efficient Agrobacterium-mediated Transformation System on Japonica Rice

doi:10.3321/j.issn:1000-7091.2005.03.003

Abstract

Abstract: A few of important factors have been investigated on influencing the frequency of rice Agrobacterium-mediated transformation by using 5 Japonica cultivars. The research showed that Agrobacterium tumefaciens strain EHA105 was more efficient than that of LBA 4404 for rice transformation, and it's transformation frequency was 25.3 percent while LBA 4404's transformation frequency was 7.6 percent. Main difference was found in frequency of inducement, transformation, differentiation of different explants and cultivars. Frequency of inducement and transformation from young embryos was obviously higher than that of mature embryos, but it's differentiation frequency was lower than that of mature embryos. Meanwhile, frequency of transformation, differentiation of different cultivars was different, which indicated rice genotype difference. And frequency of transformation, differentiation of different batches from the same cultivar was different, which showed that a few of factors influencing transformation were not yet found in rice transformation. It was proved by GUS gene expression through GUS stain of resistant callus, leaves and roots of transformation plants. Transgenic rice was testified by analysis of PCR and Southern-blotting. It showed that transformation system was reliable for Japonica rice.

Key words: Agrobacterium tumef aciens, Transgene, Japonica rice

CLC Number:

S511.2+2.035.3

PEI Zhong-you, SUN Shou-jun, LI Ling, LI Ming. Study on Efficient Agrobacterium-mediated Transformation System on Japonica Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(3): 10-13. doi: 10.3321/j.issn:1000-7091.2005.03.003.

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