The Use of Bulk DNA for Analysis of Genetic Diversity in Maize Populations by SSR

doi:10.3321/j.issn:1000-7091.2005.01.002

Abstract

Abstract: By use of 90 individuals DNA from Gold Queen maize population,6 inbred lines DNA, and 15 pair SSR primers, the effect of 4 kinds of DNA sample treatments of the individual DNA sample, the mixed DNA sample with 3 individuals, with 10 individuals, and with 15 individuals for the genetic diversity in the same population were compared and analyzed. The result indicated that using individual DNA sample could get comparatively complete genetic information including allele numbers, the gene frequency, the proportion of het- erozygosity etc. It could applied to the analysis of genetic structure of a single or a few populations of maize. But this method needs much more work than the bulk sample. The use of bulk DNA sample will make the genetic information decrease. Comparison of detective result of 4 kinds of DNA sample treatments suggested that the de-tective result of the mixed DNA sample with 15 individuals had a bigger error,but utilizing the mixed DNA sample with 3-10 individuals could reflect allele numbers and difference of a maize population, because of con-sumedly reducing the experiment workload,it can be applied to the comparison for genetic difference in the more maize populations.

Key words: SSR, Maize, Population, Genetic diversity

CLC Number:

S513.01

WANG Tie-gu, CHEN Yan-hui, WU Lian-cheng, KU Li-xia, HOU Ben-jun. The Use of Bulk DNA for Analysis of Genetic Diversity in Maize Populations by SSR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(1): 6-11. doi: 10.3321/j.issn:1000-7091.2005.01.002.

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