Comparative Studies on the Soluble Protein Changes of Major Organs and Tissues in Normal and Hemorrhagic Diseased Crucian Carp

doi:10.3321/j.issn:1000-7091.2003.04.027

Abstract

Abstract: Eight kinds of tissue soluble proteins of normal and Hemorrhagic diseased crucian carp were analysed by SDS-Polyacrylamid gel electrophoresis (SDS-PAGE).The results showed that the protein electrophoretic bands of heart,liver,kidney,branch,brian and muscle of diseased crucian carp were different from those of normal ones,five bands disappeared in heart and kidney;one new band present and one band disappeared in liver; two new bands present and two bands disappeared in branch;five new bands present in muscle.It indicated that the protein metabolism of fish changed greatly after infected by pathogenic bacteria.The changes of protein can be considered as a biochemical diagnosis indexes and the basis for studying the pathogenic mechanism at the biochemistry level and early prevention.

Key words: Crucian carp, Hemorrhagic disease, Soluble protein, SDS-PAGE

CLC Number:

S943.117.41+1

XU Xin, PENG Yong-kang. Comparative Studies on the Soluble Protein Changes of Major Organs and Tissues in Normal and Hemorrhagic Diseased Crucian Carp[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(4): 99-103. doi: 10.3321/j.issn:1000-7091.2003.04.027.

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