Effects of the Chinese Herbal Compounds on the Heat Shock Protein 70 Expression of Crucian Carp

doi:10.3969/j.issn.1000-7091.2012.z1.078

Abstract

Abstract: In this study， real time quantitative PCR method was performed to understand the effects of fourkinds of Chinese herbal compounds， including AH， CD， Ⅳ and APS on the expression of heat shock protein 70 inthe liver and brain tissues of Crucian carp． A total of 60 fishes were divided into 5 groups and fed on diet with 3 %Chinese herbal compounds of body weight． After feeding for 30 days continuously， the expression of HSP 70 in theliver and brain tissues was investigated both under the optimum temperature ( 18℃ ) and heat shock temperature(28℃ ) for an hour． The results indicated that the expression of HSP 70 in control group increased in varying degreeduring the heat shock stress， and it was significantly higher in the liver than that of brain tissues both under the normal condition or heat shock stress． Of four experimental groups， the content of HSP 70 mRNA was found to increasein AH， IV and APS groups while decrease in CD group after heat shock stress， significantly decreasing in the livertissues． In addition， AH Chinese herb， with the main function of replenishing blood and Qi， could greatly enhancethe expression of HSP 70 in the liver of Crucian carp by 4． 86 folds and 3 ． 43 folds compared to control group under18℃ and 28℃ ， respectively． In conclusion， AH compound was found to promote high-level expression of HSP 70gene in Crucian carp， to be helpful to resist heat shock and could be applied as an effective anti-heat stress drug．

Key words: The Chinese herbal compounds, Crucian carp, RT-PCR, HSP 70

CLC Number:

S948

LI Shao-wu, WANG Di, SU Ling, LIU Hong-bai. Effects of the Chinese Herbal Compounds on the Heat Shock Protein 70 Expression of Crucian Carp[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(S1): 401-405. doi: 10.3969/j.issn.1000-7091.2012.z1.078.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2012.z1.078

http://www.hbnxb.net/EN/Y2012/V27/IS1/401

References

[1] 曲朦, 施晓峰, 张之文, 等．赤点石斑鱼 HSP60 基因克隆及弧菌应急前后的组织表达特性分析 [J]．海洋学报, 2011 , 33(1 ) : 111 － 120．
[2] Hariclt C, Burkart V．Heat shock protein 60: regulatoryrole on innate immune cells [J]．Cellular and MolecularLife Sciences, 2007, 64: 742 － 751 ．
[3] Basu N, Todgham A E, Ackerman P A, et al．Heat shockprotein genes and their functional significance in fish [J]．Gene, 2002, 295: 173 － 183．
[4] Arrigo A P, Landry J．Expression and function of the lowmolecular weight heat shock proteins[M]/ /Morimoto RI, Tissieres A, Georgopoulos C ( eds．) ．Biology of HeatShock Proteins and Molecular Chaperons．Cold SpringHarbor Laboratory Press, 1994: 335 － 373．
[5] Garrido C, Ottavi P, Fromentin A, et al．HSP27 as a mediator of confluence-dependent resistance to cell death induced by anticancer drugs [J]．Cancer Research, 1997，57: 2 661 － 2 667．
[6] 陈琦, 华修国 , 顾晓峰, 等．复方中草药对大鼠热应激蛋白 70mRNA 的表达影响[J]．上海交通大学学报:农业科学版, 2006, 24(1 ) : 61 － 64．
[7] Shyu W C, Kao M C, Chou W Y, et al．Heat shock modulates prion protein expression in human NT-2 Cells [J]．Molecular Neuroscience, 2000, 2: 771 － 774．
[8] Vainberg I E, Dower K, Rosbash M．Nuclear export of heatshock and non-heat-shock mRNA occurs via similar pathways [J]．Molecular and Cellular Biology, 2000, 20: 3996－ 4005．
[9] Saavedra C A, Hammell C M, Heath C V, et al．Yeast heatshock mRNAs are exported through a distinct pathway defined by Rip1 p [J]．Genes and Development, 1997, 11 :2845 － 2856．
[10] de Benedetti A, Baglioni C．Translational regulation ofthe synthesis of a major heat shock protein in HeLacells [J]．The Journal of Biological Chemistry, 1986，261 : 15800 － 15804．
[11] 李玉保, 鲍恩东, 王志亮, 等．荧光定量 RT-PCR 法检测运输应激猪热休克蛋白 mRNA 转录水平[J]．中国农业科学, 2006, 39(1 ) : 187 － 192．
[12] Mureen E C, Roland S, Karl F．Different expression patterns of heat shock proteins HSP 60 and HSP 70 in zebramussels ( Dreissena polymorpha) exposed to copper andtributyltin [J]．Aquatic Toxicology，2000，47:213 － 226．
[13] 罗义, 杨阳．缺血再灌注心肌热休克蛋白 70 的mRNA 表达与缺血时间的关系[J]．岭南心血管病杂志, 2007, 5: 364 － 368．
[14] 彭毅志．热休克蛋白 90 在缺氧心肌细胞中的表达及保护作用的研究[D]．重庆: 第三军医大学, 2007．
[15] 刘先领, 刘付友, 李桂源, 等．HSP 70 在放线菌素 D 所致肺腺癌细胞增殖抑制和凋亡中的作用 [J]．生物化学与生物物理进展, 2007, 34(1 ) : 42 － 49．
[16] 张立国 , 张琚．实时定量 PCR 技术的介绍 [J]．生物技术, 2003, 13(2) : 39 － 40．
[17] 雷蕾．肉鸡组织的热应激损伤与 Hsp90 水平及其mRNA 表达的变化[D]．南京: 南京农业大学, 2008．
[18] 万文菊, 石存斌, 王纪亭, 等．热应激对剑尾鱼 HSP 70家族两成员基因表达的影响 [J]．中国水产科学，2006, 13(6) : 929 － 935．
[19] 程远芳, 宋代军, 曽有权, 等．中草药添加剂对生长猪抗热应激机理研究[J]．动物营养学报, 2006, 18 (1 ) :43 － 48．
[20] 陈以意, 徐国忠, 张克春．中草药添加剂对热应激奶牛生产性能及生理指标的影响[J]．乳业科学与技术, 2010, 1 : 39 － 41 ．
[21] 宋晓琳, 石达友, 郭世宁．抗蛋鸡热应激的中药研究进展[J]．中国兽医杂志, 2010, 46(9) : 62 － 63．
[22] 萨姆布鲁克, D W 拉塞尔．分子克隆试验指南[M]．第三版．北京: 科学出版社, 2002: 522 － 525．
[23] 张洪德, 张庆茹, 杜健, 等．加减白虎汤对奶牛热应激蛋白的影响[J]．黑龙江畜牧兽医: 科技版, 2010，6: 135 － 136．
[24] Glover J R, Lindquist S．HSP104, HSP70, and HSP40: anovel chaperone system that rescues previously aggregated proteins [J]．Cell, 1998, 94: 73 － 82．
[25] Kilgore J L, Tmson B F, Saunders D K, et al．Stress protein induction in skeletal muscle comparison of laboratory models to naturally occurring hypertrophy [J]．J ApplPhysiol, 1994, 76(2) : 598 － 601 ．
[26] Nagata K, Satoh M, Miller A D, et al．Involvement of Hsp47 in the folding and processing of procollagen in the endoplasmic reticulum [A]．Molecular Chaperones in theLife Cycle of Proteins [M]．New York: Dekker, 1998，10: 225 － 240．

Please choose a citation manager

Content to export