QTL Mapping of Soluble Protein Content in Soybean by Two Tail Analysis

doi:10.7668/hbnxb.2014.04.013

Abstract

Abstract: Water soluble protein is the main component of soybean seed protein,the percentage of water soluble protein is an important index to value measure and quality evaluation.Two tail analysis in three selection pressure(30%,20%,10%)were employed to detect quantitative trait loci(QTL)of soybean soluble protein content using 188 recombination inbred lines(RIL)population derived from a cross between Jidou 12 and Heidou,Jidou 12 has high SPC(38.70±1.32)% and Heidou has low SPC(32.40±1.08)%.As a result,SPC phenotypic was appeared to be normal transgressive segregation distribution.And 23 QTLs were detected in 3 selection pressure(30%,20%,10%),distribution on A1,A2,C2,D1b,D2,E,F,H,I,K,L,M and O linkage group,13 of those favorable genes from Jidou 12.There were 2 QTL qSPC-6-1, qSPC-8-1 were detected by the two years in 10% selection pressure.The linkage marker of qSPC-6-1 was Sat_062,in two years genetic contribution is 9.78% and 9.66% respectively and favorable gene from Jidou12;The linkage marker of qSPC-8-1 was Satt177,in two years genetic contribution is 9.78% and 7.98% respectively and favorable gene from Heidou.These studies provide a theoretical basis for marker-assisted breeding.

Key words: Soybean, Soluble protein, Two tail analysis, QTL

CLC Number:

S565.03

CHEN Qiang, LEI Ya-kun, YAN Long, DENG Ying-ying, YANG Chun-yan, QIAO Ya-ke, LIU Bing-qiang, LIU Qing, TANG Xiao-dong, ZHANG Meng-chen. QTL Mapping of Soluble Protein Content in Soybean by Two Tail Analysis[J]. journal1, 2014, 29(4): 80-86. doi: 10.7668/hbnxb.2014.04.013.

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