Abstract: The composition of different fundamental substrates and the different concentration of the hormone for the cotyledon of the Trifolium repens and its hypocotyls taken as the explants were studied. The results show: the strongest differentiation ability of the cellulae on the top of the hypocotyls was more than 65% of the differentiation. The same ability of the cotyledon was right behind it. The rest parts of the hypocotyls have rarely the ability. The higher concentrated vitamins and the minor elements could raise about 15% of the regeneration frequency of the cotyledon, but they have no influence on the top cellulae of the hypocotyls. When the concentration was 6-BA 3 mg/L+NAA 0.08 mg/L the regeneration frequency of the top hypocotyls was more than 80%.

Key words: Trifolium repens, Explant, Regeneration

CLC Number: 

• S541+20.5