Abstract: A Northern China cucumber inbred line L04-203 was used as plant material.Through systematic study on explant type,adventitious shoot induction medium and shoot elongation medium,an efficient cucumber somatic regeneration system was established.The results indicated that,cotyledonary node with a little bit of hypocotyl was the best explants.Ratio of BA/NAA in the medium has a remarkable influence on regeneration rate and callus induction.Shoots were directly initiated in the incision when NAA concentration was 0 or very low,callus in the incision increased as NAA concentration increased.The optimum induction medium was MS+BA/NAA(2/0.2 mg/L).3.8 shoots can be obtained per explant.The optimum shoot elongation medium was MS+BA 0.5 mg/L.

Key words: Cucumber(Cucumis sativus L.), Cotyledonary node, Regeneration system

CLC Number: 

• S642.2