Study of Monoclonal Antibodies Sandwich LAB-ELISA Detecti ng Pseudorabies Virus

doi:10.3321/j.issn:1000-7091.2001.01.024

Abstract

Abstract: A sandwich LAB-ELISA was developed to detect Pseudorabies virus (PrV) on the basis of species specific monoclonal antibodies in this study.The results showed that there was no cross reactions between the PrV and other common pathogens.The minimum concentration of the antigen that could be detected with this assay was 8.9μg/mL.The most suitable organ to get samples was brain or tonsil.The positive rates to artificially infected rabbits,naturally infected swine and clinical dubious swine were 75%,75% and 72.7%.Therefore this assay was a suitable method with good specificity and sensitivity in the diagnosis of Pseudorabies.

Key words: Pseudorabies virus, LAB-ELISA, Detection, Diagnosis

CLC Number:

S8.3

MIAO Deyuan, YANG Bing, LI Fuqiang, ZHANG Peijun, GONG Yumei, LI Yongqing, FU Lei, GAO Peiliang. Study of Monoclonal Antibodies Sandwich LAB-ELISA Detecti ng Pseudorabies Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(1): 127-131. doi: 10.3321/j.issn:1000-7091.2001.01.024.

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