Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 1999, Vol. 14 ›› Issue (3): 103-108. doi: 10.3321/j.issn:1000-7091.1999.03.021

Special Issue: Sweet pepper； Biotechnology；

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Regeneration of Transgenic Pepper Plants Resistant to TMV and CMV and Its Resistance Assay

Bi Yuping1, Shan Lei1, Wang Xingjun1, Xu Pingli1   

  1. 1. Biologic Technology Center, Academy of Agriculture of Shandong Province, Jinan 250100;
    2. Department of Horticulture, College of Agriculture of Tianjin, Tianjin 300381;
    3. College of Biology, Chinese Agricultural University, Beijing, 100094
  • Published:1999-09-28

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Abstract: A bivalent plant expressing vector carrying TMV cp and CMV cp and CMV cp has been constructed. Transgenic plants resistant to kanamycin have been obtained from to pepper cultivars, Nong da40 and Xiangyan no.1, based on the high efficient pepper gene transformation protocol established previously. The results of molecular assay indicated that sixteen plants presented positive dot blot signals with two of which presented negative PCR signals. The results of leaf surface virus inoculation test indicated that even plants presented immunity to TMV and CMV.

Key words: Double resistance, TMV, CMV, Transgenic pepper plants

Cite this article

Bi Yuping, Shan Lei, Wang Xingjun, Xu Pingli. Regeneration of Transgenic Pepper Plants Resistant to TMV and CMV and Its Resistance Assay[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(3): 103-108. doi: 10.3321/j.issn:1000-7091.1999.03.021.

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