High Efficiency Regeneration and Transformation of Lettuce (Lactuca sativa var. capitata L.)Using Agrobacterium Vector

doi:10.3321/j.issn:1000-7091.1996.01.020

Abstract

Abstract: An efficient procedure for obtaining transgenic lettuce plants using agrobacterium is described. The young leaf sections,when cultured on MS medium with 0.5mg/L BA,0.1 mg/L NAA,had a 87% plant regeneration rate, and more than one shoot were regenerated per regenerable explant during culturing. After co-cultured will a}muacteria,the explants had a 65% regeneration rate on MS medium with 100mg/L kanamycin. These regenerated plants showed a 50% positive reaction frequency when their leaf slices incubated with X-Glu solution.

Key words: Lettuce, Regeneration, Transformation, Agrobacterium

CLC Number:

S636.201

Liu Fan, Li Yan, Cao Mingqing. High Efficiency Regeneration and Transformation of Lettuce (Lactuca sativa var. capitata L.)Using Agrobacterium Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1996, 11(1): 109-113. doi: 10.3321/j.issn:1000-7091.1996.01.020.

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