Identification of the Heterogeneity and Analysis of the Activity of Chinese Soybean Lipoxygenase

doi:10.3321/j.issn:1000-7091.1996.01.005

Abstract

Abstract: The grass-beany flavor of soybean foodstuff is related to lipoxygenase(Lox);900 soybean cultivars in 26 provinces of China have been tested with IF:F-PAGE. The results show that there are lipoxygenase mutants in Chinese soybean cultivars,most of them are the seeds lacking Lox-3, moreover, rare materials lacking Lox-1, Lox-2, even Lox-2,3. The gels of IEF-PAGE are scanned by Ultroscan Laser Densitometer. The activity of Lox isoenzymes among the different soybean breeds is distinguished because of the different peak value of Lox isoenzymes.β-carotene bleaching technique is used to test the activity of lipoxygenase in the mutant soybean which have been selected by IEF-PAGE. Thus these materials can result in developing a batch of soybean cultivars which have desirable favor with good agronomic performance. That will be beneficial for enlarging regional production and food processing.

Key words: Soybean, Lox, IEF-PAGE, Activity, Area, Bleaching ability

CLC Number:

S565.101

Fu Cuizhen, Xu Wenying, Chang Ruzhen. Identification of the Heterogeneity and Analysis of the Activity of Chinese Soybean Lipoxygenase[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1996, 11(1): 25-29. doi: 10.3321/j.issn:1000-7091.1996.01.005.

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