A Study on the Culture Medium of Regenerative Cultivation with Shoot Apex of 3 Cotton Species

doi:10.3321/j.issn:1000-7091.1988.04.009

Abstract

Abstract: Shoot apex(5-10mm in lenght)of Sea Island Cotton(Gossypium Barbadense L.),Upland Cotton(Gossypium Hirsutum L.)and Tree Cotton(Gossypium Arboreum L.)were cultured on improved MS medium supplemented with IAA and KT,all resulting in formation of complete plantlet.This way provided the possibility of micropropagation of cotton clones derived from the shoot apex.The improved MS culture medium is applicable not only to the cultivation of cotton diploid with shoot apex,but also to the cultivation of cotton haploid with shoot apex.It provided possidility for the fast breeding of haploid.Shoot apex and axillary bud in the early stage of the development of cotton plandet are easier to strike roots than those in the bud and boll sqaring stage,and their roots,shoot and leaf differentiation are also better.The temperature 32℃ in daytime and the temperature 28℃ in nighttime aremore favorable to root striking and differentiation of shoot and leaf than other temperatures in the experiment.

Key words: Gossypium barbadense L., Gossypium hirsutum L., Gossypium arboreum L., Shoot apical culture, Improved MS medium

Jia Jingri, Xu Rongqi, Liu Jinxing. A Study on the Culture Medium of Regenerative Cultivation with Shoot Apex of 3 Cotton Species[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1988, 3(4): 45-49. doi: 10.3321/j.issn:1000-7091.1988.04.009.

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