玉米CMS-C不育系及保持系差异基因的克隆与表达分析

doi:10.7668/hbnxb.2014.03.003

华北农学报 ›› 2014, Vol. 29 ›› Issue (3): 11-15. doi: 10.7668/hbnxb.2014.03.003

所属专题： 玉米；生物技术；

玉米CMS-C不育系及保持系差异基因的克隆与表达分析

王继玥, 易洪杨, 汪生庆, 曹墨菊

四川农业大学玉米研究所, 教育部作物基因资源与遗传改良重点实验室, 农业部西南玉米生物学及遗传育种重点实验室, 四川成都 611130

收稿日期:2014-03-13 出版日期:2014-06-28
通讯作者: 曹墨菊（1965-），女，河北魏县人，教授，博士，主要从事玉米雄性不育研究。
作者简介:王继玥（1984-），男，四川南充人，在读博士，主要从事玉米雄性不育研究。王继玥、易洪杨为同等贡献作者。
基金资助:
国家自然科学基金项目（30971794）

Cloning and Expression Analysis of Differential Genes Between C-Type Cytoplasmic Sterile Line and Its Maintainer Line in Maize

WANG Ji-yue, YI Hong-yang, WANG Sheng-qing, CAO Mo-ju

Maize Research Institute, Sichuan Agricultural University, Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu 611130, China

Received:2014-03-13 Published:2014-06-28

摘要/Abstract

摘要： 以玉米不育系C48-2及保持系N48-2为材料，利用PCR和RT-PCR技术克隆了嵌合orf118-b 并研究了 cox2 转录本的结构变化。orf118-b 是玉米C48-2线粒体基因组特有的嵌合 orf，并能在单核期花药中特异表达。cox2^* 仅在N48-2单核期花药中特异表达，cox2^* 是 cox2 基因转录本GRM ZM5G862955_T01的新注释，其5'UTR区域被延长。利用Real-Time qPCR检测了 cdpk 在不育系及保持系雄穗发育的花粉母细胞时期、四分体时期、单核期和双核期的基因表达水平。结果表明，cdpk 在C48-2的母细胞时期、四分体时期、双核期上调表达，在C48-2的单核期下调表达。这些发现为进一步探索玉米CMS-C核质互作导致花粉败育的分子机制提供参考。

关键词: 玉米, CMS-C, 嵌合ORF, 差异表达

Abstract: orf118-b and cox2^* were cloned from sterile line C48-2 and it maintainer line N48-2 in maize by PCR and RT-PCR.orf118-b is a specific chimeric open reading frame in mitochondria genome of CMS-C line. cox2^* was special transcript of cox2 only in N48-2, based on the maize genomic database, 5'UTR region of the transcript was extended comparison to the existed annotation.Expression of cdpk between C-Type cytoplasmic male sterility line and maintainer line at the pollen mother cells(PMCs) stage, the tetrad stage, the uninucleate stage and the binuclear stage were determined using Real-Time PCR.The results showed that cdpk was up-regulated in C48-2 at mother cell stage, tetrad stage, the binuclear stage, but was down-regulated in C48-2 at binuclear uninucleate stage.This results provide foundation for further explaining molecular mechanism of CMS-C in maize.

Key words: Maize, CMS-C, Chimeric ORF, Differential expression

中图分类号:

S513.03

王继玥, 易洪杨, 汪生庆, 曹墨菊. 玉米CMS-C不育系及保持系差异基因的克隆与表达分析[J]. 华北农学报, 2014, 29(3): 11-15. doi: 10.7668/hbnxb.2014.03.003.

WANG Ji-yue, YI Hong-yang, WANG Sheng-qing, CAO Mo-ju. Cloning and Expression Analysis of Differential Genes Between C-Type Cytoplasmic Sterile Line and Its Maintainer Line in Maize[J]. journal1, 2014, 29(3): 11-15. doi: 10.7668/hbnxb.2014.03.003.

http://www.hbnxb.net/CN/Y2014/V29/I3/11

参考文献

[1] Fujii S,Toriyama K.Genome barriers between nuclei and mitochondria exemplified by cytoplasmic male sterility[J].Plant Cell Physiol,2008,49(10):1484-1494.
[2] Rottman W H,Brears T,Hodge T P,et al.A mitochondrial gene is lost via homologous recombination during reversion of CMS T maize to fertility[J].EMBO J,1987,6(6)：1541-1546.
[3] Renate Horn,Joachim E G,Hustedt,et al.The CMS-associated 16 kDa protein encoded by orfH522 in the PET1 cytoplasm is also present in other male-sterile cytoplasms of sunflower[J].Plant Mol Biol,1996,30(3):523-538.
[4] Ssingh M,Brown G.Suppression of cytoplasmic male sterility by nuclear gene alters expression of a novel mitochondrial gene region[J].Plant Cell,1991,3(12):1349-1362.
[5] Das S,Sen S,Chakraborty A,et al.An unedited 1.1 kb mitochondrial orfB gene transcript in the wild abortive cytoplasmic male sterility(WA-CMS) system of Oryza sativa L.subsp.indica.[J].BMC Plant Biol,2010,10:39.
[6] Dangping Luo,Hong Xu,Zhenlan Liu,et al.A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice[J].Nature Genetics,2013,45(5):573-577.
[7] Xia T,Liu J.Relation between ethylene and cytoplasmic male sterility in maize(Zea mays L.)[J].Acta Agriculturae Boreali Sinica,1996,11(3):68-72.
[8] Xia T,Liu J.Cytochrome oxidase activity and ATP content of male-Sterile cytoplasm in maize(Zea mays L.)[J].Acta Agriculturae Boreali Sinica,1994,9(4):33-37.
[9] Huang L,Xiang J,Jiazhou Liu,et al.Expression characterization of genes for CMS-C in maize[J].Protoplasma,2011,249:1119 1127.
[10] Allen O,Fauron C M,Minx P,et al.Comparisons among two fertile and three male-sterile mitochondrial genomes of maize[J].Genetics,2007,177(2):1173-1192.
[11] Wang J,Cao M J,Pan G T,et al.RNA editing of mitochondrial functional genes atp6 and cox2 in maize(Zea mays L.)[J].Mitochondrion,2009,9(5):364-369.
[12] Stefan Stephane Bentolila Stefanov.A reevaluation of rice mitochondrial evolution based onthe complete sequence of male-fertile and nale-sterile mitochondrial genomes[J].Plant Physiology,2012,2(158):996 1017.
[13] Igarashi K,Kazama T,Motomura K,et al.Whole genomic sequencing of RT98 mitochondria derived from oryza rufipogon and Northern Blot analysis to uncover a cytoplasmic male sterility-associated gene[J].Plant Cell Physiol,2013,54(2):237-243.
[14] 汪静.玉米细胞质雄性不育系及其保持系线粒体功能基因RNA编辑研究[D].雅安：四川农业大学,2007：22-23.
[15] Clifton S W,Minx P,Fauron C M, et al. Sequence and comparative analysis of the maize NB mitochondrial genome[J].Plant Physiology,2004,136(3):3486-3503.
[16] Kubo T,Kitazaki K,Matsunag M,et al.Male sterility-inducing mitochondrial genomes:how do they differ[J].Critical Reviews in Plant Sciences,2011,30(4):378-400.
[17] 章国卫,宋东,陈竺.mRNA选择性剪接的分子机制[J].遗传学报,2004,31(1):102-107.
[18] 王娇娇,韩胜芳,李小娟,等.钙依赖蛋白激酶(CDPKs)介导植物信号转导的分子基础[J].草业学报,2009,18(3):241-250.
[19] 万丙良查中萍戚华雄.钙依赖的蛋白激酶与植物抗逆性[J].生物技术通报,2009(1):7-10.
[20] Gyeong M Y,Dowd P E,Gilory S. et al. Calcium-dependent protein kinase isoforms in petunia have distinctfunctions in pollen tube growth,including regulating polarity[J].Plant Cell,2006,18(4):867-878.

选择文件类型/文献管理软件名称

选择包含的内容

玉米CMS-C不育系及保持系差异基因的克隆与表达分析

Cloning and Expression Analysis of Differential Genes Between C-Type Cytoplasmic Sterile Line and Its Maintainer Line in Maize

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	翟立超, 张丽华, 郑孟静, 吕丽华, 申海平, 姚海坡, 贾秀领. 夏玉米粒位效应对增密的响应及其碳氮代谢特征[J]. 华北农学报, 2022, 37(5): 97-104.
[2]	刘帅, 徐学欣, 赵金科, 曲文凯, 郝天佳, 孟繁港, 贾靖, 赵长星. 滴灌水肥一体化下施氮量与频次对夏玉米光合特性、保护酶活性及产量的影响[J]. 华北农学报, 2022, 37(5): 114-123.
[3]	王丽娟, 刘丹, 徐永清, 冯旭, 贺付蒙, 李爱雨, 王雪, 杨燕, 李翠婷, 袁强, 李凤兰. 寒地玉米秸秆不同腐熟时期的理化性状及微生物多样性分析[J]. 华北农学报, 2022, 37(5): 132-139.
[4]	林静, 林建新, 张扬, 卢和顶, 陈山虎, 廖长见. 利用转录组解析鲜食玉米闽甜6855的耐寒机制[J]. 华北农学报, 2022, 37(5): 1-8.
[5]	王燕宁, 黄涛, 吴光亮, 黄诗颖, 钟奇, 王鹏, 杨朦朦, 李才敬, 程琴, 贺浩华, 金伟, 郭凌, 边建民. 水稻芽期响应镉胁迫的转录组分析[J]. 华北农学报, 2022, 37(5): 25-35.
[6]	付敬锋, 罗上轲, 程乙, 张军, 魏盛, 曾涛, 陈杜, 魏鹏程, 郑迎霞, 宋碧. 不同覆膜方式及施氮量对春玉米产量和氮素吸收利用的影响[J]. 华北农学报, 2022, 37(4): 158-168.
[7]	李强, 孔凡磊, 袁继超. 氮肥运筹对不同玉米品种氮素吸收、利用及田间氮平衡的影响[J]. 华北农学报, 2022, 37(4): 169-181.
[8]	赵思琪, 曲比伍合, 罗婷, 余学杰, 柯永培, 苟齐贤, 石海春. 玉米对灰斑病胁迫的生理生化响应[J]. 华北农学报, 2022, 37(4): 190-197.
[9]	王泳超, 燕博文, 曹红章, 王山聪, 马梦金, 张俊杰, 郭家萌, 王浩, 邵瑞鑫, 杨青华. DA-6与CCC复配对密植下玉米叶片光合及抗早衰能力的影响[J]. 华北农学报, 2022, 37(4): 90-102.
[10]	赵长江, 都梦翔, 宋巨奇, 徐尚缘, 贺琳, 徐晶宇, 杨克军, 李佐同. 玉米NRL基因家族鉴定与逆境表达分析[J]. 华北农学报, 2022, 37(4): 1-10.
[11]	安清明, 王星, 吴震洋, 孟金柱, 赵园园, 宋兴超. 基于转录组挖掘不同性别贵州白山羊肌肉生长发育的关键基因及PI3K/ATK信号通路分析[J]. 华北农学报, 2022, 37(3): 213-222.
[12]	张敬敬, 李冰, 史宇凡, 高秀瑞, 潘秀清, 宋雪, 武彦荣. 不同硬度西瓜果皮的转录组测序及相关基因表达分析[J]. 华北农学报, 2022, 37(3): 44-52.
[13]	张宗祥, 黄峥嵘, 吴雪凡, 刘楠楠, 李笑笑, 董召荣, 宋贺. 土壤酸化对玉米产量、氮代谢及相关基因表达的影响[J]. 华北农学报, 2022, 37(3): 94-103.
[14]	邰继承, 韩镁琪, 杨恒山, 张玉芹, 马金慧, 郭晓旭. 浅埋滴灌下尿素减量配施UAN对春玉米干物质积累及氮效率的影响[J]. 华北农学报, 2022, 37(3): 136-144.
[15]	刘建玲, 吴晶, 贾可, 廖文华, 吕英华, 马俊永. 冬小麦-夏玉米轮作区土壤磷与磷肥产量效应变化[J]. 华北农学报, 2022, 37(3): 104-111.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

玉米CMS-C不育系及保持系差异基因的克隆与表达分析

Cloning and Expression Analysis of Differential Genes Between C-Type Cytoplasmic Sterile Line and Its Maintainer Line in Maize

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价