华北农学报 ›› 2014, Vol. 29 ›› Issue (3): 6-10. doi: 10.7668/hbnxb.2014.03.002

所属专题: 葡萄

• 论文 • 上一篇    下一篇

甘蓝硫代葡萄糖苷总量性状的QTL定位及分析

田多成1,2, 何洪巨2, 严慧玲3, 颉建明1, 康俊根2   

  1. 1. 甘肃农业大学 农学院, 甘肃 兰州 730070;
    2. 北京市农林科学院 蔬菜研究中心, 农业部华北地区园艺作物 生物学与种质创制重点实验室, 北京 100097;
    3. 河北省农林科学院 经济作物研究所, 河北 石家庄 050051
  • 收稿日期:2014-03-12 出版日期:2014-06-28
  • 通讯作者: 康俊根(1972-),男,山西忻州人,研究员,博士,主要从事蔬菜分子育种研究。颉建明(1970-),男,甘肃天水人,教授,博士,主要从事园艺设施教育研究。
  • 作者简介:田多成(1987-),男,甘肃礼县人,在读硕士,主要从事蔬菜分子育种研究。
  • 基金资助:
    国家自然科学基金项目(31171958);“十二五”国家科技支撑计划项目(2012BAD02B01;2012BAD50G01);“863”项目(2012AA100105;2012AA020103)

Mapping QTL and Analysis on Glucosinolates Total Content in Cabbage

TIAN Duo-cheng1,2, HE Hong-ju2, YAN Hui-ling3, XIE Jian-ming1, KANG Jun-gen2   

  1. 1. College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
    2. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China), Ministry of Agriculture, Beijing 100097, China;
    3. Institute of Economic Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China
  • Received:2014-03-12 Published:2014-06-28

摘要: 采用HPLC方法分析了作图群体双亲及F2 83个单株的硫代葡萄糖苷总含量,在已构建的高密度甘蓝遗传图谱的基础上,运用MapQTL 4.0软件对结球甘蓝F2群体硫代葡萄糖苷总含量进行QTL定位和分析。最终检测到3个控制甘蓝硫代葡萄糖苷含量性状的QTLs(分别位于LG7上的 qGS-7-1 和位于LG8上的 qGS-8-1qGS-8-2),其贡献率分别为13.6%,7.5%,5.6%。并得到同时控制硫代葡萄糖苷含量QTLs共分离的分子标记me27-em25-2和E38M55-7,均可为辅助选育富含硫代葡萄糖苷甘蓝品种提供理论依据。

关键词: 结球甘蓝, 硫代葡萄糖苷, 数量性状位点

Abstract: Glucosinolates, anticancer active substances, are widely present in Brassica crops.It will be of important significance to carry out the breeding of high content of Glucosinolates new varieties of cabbage.This study analyzed total glucosinolate content of F2 mapping population parents and that of 83 individual plants by HPLC methods.Based on the cabbage high-density genetic linkage map which had been constructed previously, QTL mapping and analysis related to Glucosinolates total content in the individuals of F2 were performed using MapQTL 4.0.Three QTLs(qGS-7-1, qGS-8-1, qGS-8-2) located on LG7 and LG8 controlling the traits of Glucosinolates total content in cabbage were detected.The contribution rate were 13.6%, 7.5% and 5.6%, respectively.Meanwhile, two molecular markers(me27-em25-2, E38M55-7) coseparating with the QTLs could be useful for marker assisted selection of cabbage breeding rich in Glucosinolates.

Key words: Brassica oleracea var. capitata, Glucosinolates total content, QTL

中图分类号: 

引用本文

田多成, 何洪巨, 严慧玲, 颉建明, 康俊根. 甘蓝硫代葡萄糖苷总量性状的QTL定位及分析[J]. 华北农学报, 2014, 29(3): 6-10. doi: 10.7668/hbnxb.2014.03.002.

TIAN Duo-cheng, HE Hong-ju, YAN Hui-ling, XIE Jian-ming, KANG Jun-gen. Mapping QTL and Analysis on Glucosinolates Total Content in Cabbage[J]. journal1, 2014, 29(3): 6-10. doi: 10.7668/hbnxb.2014.03.002.

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