华北农学报 ›› 2013, Vol. 28 ›› Issue (4): 23-30. doi: 10.3969/j.issn.1000-7091.2013.04.005

所属专题: 水稻

• 论文 • 上一篇    下一篇

利用染色体单片段代换系定位水稻穗粒数QTL

朱金燕1, 杨梅2, 王中德2, 王军1, 周勇2, 杨杰1, 范方军1, 李文奇1, 梁国华; 仲维功2   

  1. 1. 江苏省农业科学院粮食作物研究所,国家水稻改良中心南京分中心, 江苏南京 210014;
    2. 扬州大学教育部植物功能基因组学重点实验室, 江苏扬州 225009
  • 收稿日期:2013-04-27 出版日期:2013-08-28
  • 通讯作者: 仲维功(1954- ),男,江苏兴化人,研究员,主要从事水稻遗传育种研究 梁国华(1965- ),男,江苏盱眙人,研究员,主要从事水稻分子遗传育种研究
  • 作者简介:朱金燕(1983- ),女,江苏南通人,助理研究员,博士,主要从事水稻遗传育种研究 朱金燕 杨梅为同等贡献作者
  • 基金资助:
    国家与江苏省粮食丰产科技工程项目(No.2011BAD16B03;BE2009425);优质高产多抗广适性水稻新品种选育项目(CX(12)1003-2);高等学校博士学科点专项科研基金项目(20103250110004;20113250120001);江苏省大学生实践创新训练计划项目(2012JSSPITP1403)

Identification of Grain Number Quantitative Trait Loci with Single Segment Substituted Lines in Rice

ZHU Jin-yan11, YANG Mei2, WANG Zhong-de2, WANG Jun1, ZHOU Yong2, YANG Jie1, FAN Fang-jun1, LI Wen-qi1, LIANG Guo-hua2, ZHONG Wei-gong1   

  1. 1. Nanjing Branch of Chinese National Center for Rice Improvement, Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. Jiangsu Provincial Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2013-04-27 Published:2013-08-28

摘要: 以85个单片段代换系为材料,其受体亲本为广陆矮4号,供体亲本为日本晴。通过单因素方差分析和Dunnett’s多重比较,分析单片段代换系与受体亲本之间穗粒数的差异,对代换片段上的穗粒数QTL进行鉴定。以P≤0.001为阈值共检测到27个穗粒数QTLs,分布于除第10染色体外的其他11条染色体上。其中,2个QTLs的加性效应表现为减效作用,另外25个穗粒数QTLs的加性效应表现为增效作用。其加性效应值的变化范围为-37.5~96.9,加性效应百分率的变化范围为-29.07%~88.57%。为进一步发掘和利用新的水稻穗粒数QTL奠定基础。

关键词: 水稻, 穗粒数, 数量性状位点, 单片段代换系, 代换作图

Abstract: Grain number is one of the important components of rice yield which is a typical quantitative trait and can easily be influenced by genetic background and environmental factors.Single segment substitution lines (SSSLs) can effectively reduce the interference of the genetic background.We developed a set of genome- wide SSSLs carrying Nipponbare introgression segments in the background of Guangluai 4.Quantitative trail loci (QTL) analysis involved one- way ANOVA and Dunnett' s test for comparing SSSLs with the recurrent parent Guangluai 4 (P 0.001).We detected 27 QTLs for grain number distributed on 11 rice chromosomes except chromosome 10,of which 2 had negative effect and the others positive effect.The additive effect of these QTLs ranged from-37.5 to 96.6,with additive effect percentages-29.07% to 88.57%.These results provide useful information for exploring and using new grain number related resources.

Key words: Rice, Grain number, Quantitative trait loci, Single segment substitution lines, Substitution mapping

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引用本文

朱金燕, 杨梅, 王中德, 王军, 周勇, 杨杰, 范方军, 李文奇, 梁国华; 仲维功. 利用染色体单片段代换系定位水稻穗粒数QTL[J]. 华北农学报, 2013, 28(4): 23-30. doi: 10.3969/j.issn.1000-7091.2013.04.005.

ZHU Jin-yan1, YANG Mei, WANG Zhong-de, WANG Jun, ZHOU Yong, YANG Jie, FAN Fang-jun, LI Wen-qi, LIANG Guo-hua, ZHONG Wei-gong. Identification of Grain Number Quantitative Trait Loci with Single Segment Substituted Lines in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 23-30. doi: 10.3969/j.issn.1000-7091.2013.04.005.

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