利用染色体单片段代换系定位水稻垩白QTL

doi:10.7668/hbnxb.2015.01.001

摘要/Abstract

摘要： 为了鉴定和定位水稻垩白相关QTL,分析其遗传效应,利用籼稻品种广陆矮4号为受体,粳稻品种日本晴为供体构建的84个染色体单片段代换系群体为试验材料,通过单因素方差分析和Dunnett's多重比较,对代换片段上垩白相关QTL进行鉴定。以P≤0.001为阈值,共检测到36个垩白相关QTL。其中,垩白度QTL共19个,其加性效应值为-6.44~12.86,加性效应百分率为-34.04%~68.02%。垩白粒率相关QTL共17个,其加性效应值为-7.32~3.63,加性效应百分率为-8.07%~4.00%。这些QTL的鉴定为进一步精细定位并克隆相应QTL,提高稻米外观品质奠定了基础。

关键词: 水稻, 垩白度, 垩白粒率, 数量性状位点, 染色体单片段代换系, 代换作图

Abstract: In order to evaluate and mapping QTL associated with Chalkiness and then analysis their genetic effects, 84 chromosome single segment substitution lines (CSSSLs) were derived from the cross of Guangluai 4 as recipient line and Nipponbare as donor line.One-way ANOVA and Dunnett's test were employed to detect 36 QTLs (P≤0.001) associated with chalkiness, in which 19 were degree of endosperm chalkiness (DEC) related QTLs.The additive effects ranged from-6.44 to 12.86, and the additive effect contributions were-34.04%-68.02%.The other 17 QTLs were associated with percentage of grain with chalkiness (PGWC).The additive effects of them were-7.32-3.63 and the additive effect contribution were-8.07%-4.00%.These results provided useful information for fine mapping and cloning related QTLs, and will facilitate the improvement of rice appearance quality.

Key words: Rice, Degree of endosperm chalkiness, Percentage of grain with chalkiness, Quantitative trait loci, Chromosome single segment substitution lines, Substitution mapping

中图分类号:

S511.03

陶亚军, 徐梦彬, 王飞, 陈达, 周勇, 梁国华. 利用染色体单片段代换系定位水稻垩白QTL[J]. 华北农学报, 2015, 30(1): 1-8. doi: 10.7668/hbnxb.2015.01.001.

TAO Ya-jun, XU Meng-bin, WANG Fei, CHEN Da, ZHOU Yong, LIANG Guo-hua. QTL Mapping of Chalkiness Using Chromosome Single Segment Substituted Lines in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 1-8. doi: 10.7668/hbnxb.2015.01.001.

http://www.hbnxb.net/CN/Y2015/V30/I1/1

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