华北农学报 ›› 2013, Vol. 28 ›› Issue (2): 209-213. doi: 10.3969/j.issn.1000-7091.2013.02.037

所属专题: 烟草 土壤肥料 生物技术

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不同基因型烤烟根区土壤微生物和酶活性研究

刘巧真1,2, 郭芳阳1,2, 吴照辉1,2, 李芳芳3, 梁涛1,2, 曹华民1,2   

  1. 1. 河南省农业科学院 烟草研究中心, 河南 许昌 461000;
    2. 河南省烟草公司 烟草研究所, 河南 许昌 461000;
    3. 河南省烟草公司 信阳市分公司, 河南 信阳 464100
  • 收稿日期:2012-10-12 出版日期:2013-04-28
  • 通讯作者: 郭芳阳(1965-),男,河南开封人,副研究员,主要从事烟草栽培技术研究。
  • 作者简介:刘巧真(1982-),女,河南开封人,助理研究员,硕士,主要从事烟草生理生化及栽培技术研究。
  • 基金资助:
    河南省烟草公司项目(HYKJ200904)

Study on Soil Microorganism and Enzyme Activity in Root-zone of Different Flue-cured Tobaccogenotypes

LIU Qiao-zhen1,2, gUO Fang-yang1,2, WU Zhao-hui1,2, LI Fang-fang3, LIANG Tao1,2, CAO Hua-min1,2   

  1. 1. Tobacco Research Center, Henan Academy of Agricultural Sciences, Xuchang 461000, China;
    2. Tobacco Research Institute of Henan Tobacco Company, Xuchang 461000, China;
    3. Xinyang Tobacco Company of Henan Province, Xinyang 464100, China
  • Received:2012-10-12 Published:2013-04-28

摘要: 为了研究不同基因型烤烟根区土壤微生物种群数量和酶活性差异,以中烟100、NC297和KRK26为材料,研究了其根区土壤主要微生物种群数量和酶活性的变化规律。结果表明:3个基因型烤烟根区土壤细菌、真菌、放线菌、解磷菌数量随生育期变化规律基本一致,但现蕾期和采收结束均表现为KRK26根区的土壤真菌和解磷菌数量明显高于其他2个品种。根区土壤解钾菌、硝化细菌和纤维素分解菌变化规律和数量在烤烟基因型间差异明显,中烟100土壤解钾菌数量呈倒"V"型变化,现蕾期最高(53.36×104个/g),明显高于其他2个品种|NC297团棵期根区土壤硝化细菌数量最高,之后逐渐降低,而中烟100表现为先升高后下降,现蕾期最高|整个生育期中烟100根区土壤纤维素分解菌数量明显高于KRK26和NC297。NC297根区土壤过氧化氢酶活性在整个生育期均低于KRK26和中烟100,根区土壤转化酶活性则表现为中烟100明显高于KRK26和NC297。

关键词: 烤烟, 基因型, 土壤微生物, 土壤酶活性

Abstract: Zhongyan 100,NC297 and KRK26 were selected as test materials to study the soil microorganism and enzyme activity in root-zone of different flue-cured tobaccogenotypes. The results showed that the amounts of root-zone soil microphyte,epiphyte,actinomyces and phosphate-dissolving bacteria had the similar changes along with thegrowth stages,but the amounts of epiphyte and phosphate-dissolving bacteria at squaring and harvest stages were significantly higher in root-zone of KRK26 than in Zhongyan 100 and NC297. The amounts of root-zone soil po-tassium-dissolving bacteria,nitrobacteria and celluose-decomposing bacteria changed differently in different flue-cured tobaccogenotypes. The amount of potassoium-dissolving bacteria in Zhongyan 100 ascended first,and then de-scended,reaching to the highest(53. 36×104cell/g of dry soil)at squaring stage. The amount of root-zone soil nitro-bacteria in NC297 reached to the highest at rosette stage,and then decreasedgradually,while in Zhongyan 100,it ascended first,and then descended,reaching to the highest at squaring stage. During the wholegrowth period,the a-mount of root-zone soil celluose-decomposing bacteria was higher in Zhongyan 100 than in KRK26 and NC297,and the root-zone soil catalase activity was the lowest in NC297,while the root-zone soil sucrase activity was the highest in Zhongyan 100.It was concluded that the root-zone soil microorganism amounts and enzyme activities were differ-ent in different flue-cured tobaccogenotypes.

Key words: Flue-cured tobacco, Genotype, Soil microorganism, Soil enzymes activity

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引用本文

刘巧真, 郭芳阳, 吴照辉, 李芳芳, 梁涛, 曹华民. 不同基因型烤烟根区土壤微生物和酶活性研究[J]. 华北农学报, 2013, 28(2): 209-213. doi: 10.3969/j.issn.1000-7091.2013.02.037.

LIU Qiao-zhen,gUO Fang-yang, WU Zhao-hui, LI Fang-fang, LIANG Tao, CAO Hua-min,. Study on Soil Microorganism and Enzyme Activity in Root-zone of Different Flue-cured Tobaccogenotypes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 209-213. doi: 10.3969/j.issn.1000-7091.2013.02.037.

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