<i>BPGM</i>下调对静原鸡成肌细胞生长发育的影响

doi:10.7668/hbnxb.20194625

摘要/Abstract

摘要：

旨在探讨二磷酸甘油酸变位酶(BPGM)对鸡成肌细胞生长发育的影响。对宁夏大学动物科技学院张娟课题组前期转录组学和蛋白组学数据进行联合分析,筛选出可能影响生长发育的差异表达基因,探究其对成肌细胞增殖、凋亡和相关标志基因表达水平的影响,并对糖酵解通路上下游基因的表达进行定量分析。ELISA检测肌苷酸(IMP)含量;比色法检测腺苷三磷酸(ATP)含量,深入揭示关键候选基因对静原鸡肌肉发育和肉质风味的重要作用。结果表明,静原鸡胸肌与腿肌间共鉴定到差异表达基因/蛋白139个,其中在转录组和蛋白组同时上调的差异基因/蛋白75个,在转录组和蛋白组同时下调的差异基因/蛋白45个,在转录组上调蛋白组下调的差异基因/蛋白13个,在蛋白组上调转录组下调的差异基因/蛋白6个;从糖酵解/糖异生通路筛选出7个差异表达基因,分别是BPGM、磷酸甘油酸激酶1基因(PGK1)、葡萄糖-6-磷酸异构酶基因(GPI)、磷酸甘油酸变位酶基因(PGAM1)、乳酸脱氢酶A基因(LDHA)、乳酸脱氢酶B基因(LDHB)、磷酸丙糖异构酶1基因(TPI1),结合KEGG通路富集分析和蛋白网络互作分析筛选出差异表达基因BPGM。BPGM在静原鸡母鸡组织中均有表达,在胸肌中的表达量最高,且在成肌细胞分化过程中呈上调趋势。用分离培养的原代成肌细胞进行细胞转染,干扰BPGM抑制了成肌细胞的增殖而促进细胞凋亡,并调控糖酵解通路上下游基因的表达,同时抑制了IMP和ATP的生物合成。综上所述,下调BPGM可以抑制成肌细胞的生长发育及IMP和ATP的生物合成。

关键词: 静原鸡, BPGM, 糖酵解, 成肌细胞, 生长发育, 肉质风味

Abstract:

The aim of this study was to investigate the effect of bisphosphoglycerate mutase(BPGM)on the growth and development of chicken myogenic cells.The transcriptomics and proteomics data was jointly analyzed in the preliminary stage of the study to identify the differentially expressed genes that might affect the growth and development,and investigated their effects on the proliferation,apoptosis and expression levels of related marker genes of myogenic cells.Inosinic acid(IMP)was detected by ELISA and adenosine triphosphate(ATP)was detected by colorimetric assay,which revealed the important roles of the key candidate genes on muscle development and meat flavor of Jingyuan chickens.The results showed that a total of 139 differentially expressed genes/proteins were identified between the breast muscle and leg muscle of Jingyuan chickens,including 75 differentially expressed genes/proteins that were up-regulated in both the transcriptome and proteome,45 differentially expressed genes/proteins that were down-regulated in both the transcriptome and proteome,13 differentially expressed genes/proteins that were down-regulated in the transcriptome,and 6 differentially expressed genes/proteins that were up-regulated in the proteome and down-regulated in the transcriptome.7 differentially expressed genes were screened from the glycolysis/glycolysis pathway,which were BPGM, phosphoglycerate kinase 1 gene(PGK1),glucose-6-phosphate isomerase gene(GPI),phosphoglycerate mutase 1 gene(PGAM1),lactate dehydrogenase A gene(LDHA),lactate dehydrogenase B gene(LDHB)and phosphoglycerate isomerase 1 gene(TPI1),and screened for the differentially expressed gene BPGM in combination with KEGG pathway enrichment analysis and protein network interactions analysis.BPGM was expressed in all tissues of Jingyuan hens,the highest expression was in pectoral muscle,and it showed an up-regulation trend during the differentiation of myoblasts.Primary myogenesis cells were isolated and cultured for cell transfection.Interfering with BPGM inhibited myoblast proliferation and promoted apoptosis;it also affected genes upstream and downstream of the glycolytic pathway,and inhibited the biosynthesis of IMP and ATP at the same time.In conclusion,down-regulation of BPGM inhibited the growth and development of myoblasts,as well as the biosynthesis of IMP and ATP.

Key words: Jingyuan chicken, BPGM, Glycolysis, Myogenic cells, Growth and development, Meat flavor

付希, 赵薇, 禹保军, 张统, 陈亚飞, 毛向媛, 魏传浩, 马小萍, 张心雨, 张娟. BPGM下调对静原鸡成肌细胞生长发育的影响[J]. 华北农学报, 2024, 39(2): 219-230. doi: 10.7668/hbnxb.20194625.

FU Xi, ZHAO Wei, YU Baojun, ZHANG Tong, CHEN Yafei, MAO Xiangyuan, WEI Chuanhao, MA Xiaoping, ZHANG Xinyu, ZHANG Juan. The Effect of Down-regulation of BPGM on the Growth and Development of Myogenic Cells in Jingyuan Chickens[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(2): 219-230. doi: 10.7668/hbnxb.20194625.

http://www.hbnxb.net/CN/Y2024/V39/I2/219

图/表 8

表1 si-BPGM靶序列

Tab.1 si-BPGM target sequences

基因 Gene	序列名称 Sequence name	靶序列 Target sequence
BPGM	si-BPGM-1	GGCATCTCTGACAAGGACA
	si-BPGM-2	CAATGACCGTCGATACAAA
	si-BPGM-3	GGCTCTGAATCATGGAGAA

表2 RT-qPCR 引物信息

Tab.2 The RT-qPCR primers information

基因 Gene	登录号 Accession No.	序列(5'—3') Sequence(5'—3')	产物长度/bp Product length
ACTB	L08165.1	F:ATGGACTCTGGTGATGGTGTTAC	185
		R:TCGGCTGTGGTGGTGAAG
BPGM	NM_001030768.2	F:GTGCTTGATAGACTCCTTCC	135
		R:CTTGTCAGAGATGCCTTCC
Cyclin E	BI390175	F:GGTTGTTGGCATCAGTAAAG	214
		R:TATTACCTCATCTTCCTCGTTG
Cyclin E	NM_205381.2	F:CAGAAGTGCGAAGAGGAAGT	189
		R:GATGGAGTTGTCGGTGTAAAT
PCNA	NM_204170.3	F:GACTCCTCGCACGTCTCCCT	161
		R:TCCGCATTGTCTTCTGCTCTG
CDK1	NM_205314.2	F:TGCAAGGCTGTACCTCATT	174
		R:ACTCTTAACACGCGAACGA
BAK1	NM_001030920.3	F:GTTCGACAGCGGCATTA	158
		R:TGCCTTGCTGGTAGACG
FAS	NM_001199487.2	F:GTTCGTCATCACCGTCTATCG	215
		R:TTCGTAGGCTCCTCCCATC
BID	NM_204552.3	F:TCACAGGCAGTGGAAGGA	196
		R:TGTGGAAGTGTTGGCTGAT
Caspase9	BG711928	F:CGAAGGAGCAAGCACGA	187
		R:AGGTTGGACTGGGATGGAC
PGM1	NM_001038693.3	F:AACCACCTCAAGATTCGC	212
		R:GCAGCTCCAAAGTCGTAC
PFKM	NM_204223.2	F:TGGCTGTGCTCAATGTGG	244
		R:GTGGCGCTGATTTCCTC
TPI1	NM_205451.1	F:GAAGATGAACGGCGACAA	236
		R:CCCATGCAGCTCCAATAT
PGK1	NM_204985.4	F:GATGTGAAGGGCAAGCG	279
		R:CTCCACCTCAGGACCAA
GAPDH	NM_204305.2	F:TGCCGTCTGGAGAAACC	160
		R:CAGCACCCGCATCAAAG
ENO2	NM_204876.2	F:TCCTGCTCAAAGTCAACC	123
		R:TGAAAGTGTCTTCGGTCTC
ENO3	NM_205119.1	F:TGGACGGCACCGAGAAT	155
		R:CGGGACAGGCAGGATGA
PKM2	NM_205469.2	F:CACGGCACCCACGAGTA	210
		R:GGCATTGTCCAGCGTCA

图1 转录和蛋白联合分析及差异基因的筛选 A.共有差异表达基因/蛋白对应关系九象限图:Cor-up.红色圆点表示转录和蛋白共上调,Cor-down.绿色圆点表示转录和蛋白共下调,Opposite-sig.黄色圆点表示转录上调但蛋白下调,蓝色圆点表示转录下调但蛋白上调;B.差异表达基因/蛋白所富集的KEGG通路;C.差异表达基因/蛋白所富集的GO条目;D.差异表达基因/蛋白BPGM的蛋白网络互作;E.G/G通路:紫色途径为糖酵解,红色途径为糖异生,绿色为BPGM所在位置。

Fig.1 Joint transcript and protein analysis and differential gene screening A.Nine-quadrant plot of total differentially expressed gene/protein correspondences:Cor-up.Red dots indicate transcriptional and protein co-regulation;Cor-down.Green dots indicate transcriptional and protein co-regulation;Opposite-sig.Yellow dots indicate transcriptional up-regulation but protein down-regulation,blue dots indicate transcriptional down-regulation but protein up-regulation;B.KEGG pathways enriched by differentially expressed genes/proteins;C.GO entries enriched by differentially expressed genes/proteins;D.Protein network interactions of differentially expressed genes/proteins BPGMs;E.G/G pathway:Purple pathway for glycolysis,red pathway for gluconeogenesis,green for the location of BPGM.

图2 鸡BPGM基因的表达分析 A.BPGM基因在母鸡组织中的表达分析;B.BPGM基因在成肌细胞分化中的表达分析。不同小写字母表示差异显著(P<0.05),不同大写字母表示差异极显著(P<0.01)。图3—6同。

Fig.2 Expression analysis of chicken BPGM gene A.Expression analysis of BPGM gene in hen tissues;B.Expression analysis of BPGM gene in differentiation of adult myoblasts.Different lowercase letters indicate significant differences(P<0.05)and different uppercase letters indicate highly significant differences(P<0.01).The same as Fig.3—6.

图3 成肌细胞免疫荧光鉴定及细胞干扰效率检测 A.鸡原代成肌细胞第1~3 天状态;B.F₁成肌细胞免疫荧光鉴定;C.si-BPGM浓度为50 nmol/L转染荧光;D.不同浓度不同干扰序列si-BPGM的干扰效率。

Fig.3 Immunofluorescence identification of myofibroblasts and detection of cellular interference efficiency A.Chicken primary myoblasts on day 1—3;B.Immunofluorescence identification of F₁ generation myoblasts;C.Fluorescence of si-BPGM transfected at 50 nmol/L;D.Transfection efficiency of si-BPGM with different concentrations of different interference sequences.

图4 干扰BPGM对成肌细胞增殖的影响 A.EdU 检测干扰BPGM对成肌细胞增殖的影响;B.EdU阳性细胞所占百分比;C.CCK-8试验检测干扰BPGM后不同时间段对成肌细胞增殖的影响;D.转染48 h检测成肌细胞增殖标志基因的表达量。

Fig.4 Effects of interfering with BPGM on the proliferation of adult myoblasts A.EdU assay to detect the effect of interference with BPGM on the proliferation of myoblasts;B.Percentage of EdU-positive cells;C.CCK-8 assay to detect the effect of interference with BPGM on the proliferation of myoblasts at different time periods after the interference with BPGM;D.Transfection for 48 h to detect the expression of myoblasts proliferation marker genes.

图5 干扰BPGM对成肌细胞凋亡的影响 A.Annexin V-mCherry/SYTOX Green检测干扰BPGM对成肌细胞凋亡的影响;B.SYTOX Green阳性细胞所占百分比;C.转染48 h检测成肌细胞凋亡标志基因的表达量。

Fig.5 Effect of interfering BPGM on apoptosis of myoblasts A.Effect of interference with BPGM on apoptosis of myoblasts detected by Annexin V-mCherry/SYTOX Green; B.Percentage of SYTOX Green-positive cells;C.Expression of apoptosis marker genes in myoblasts detected by transfection for 48 h.

图6 干扰BPGM对成肌细胞糖酵解以及IMP、ATP含量的影响 A.干扰BPGM基因后糖酵解通路中上下游基因的表达量;B.干扰BPGM对成肌细胞内IMP含量的影响;C.干扰BPGM对成肌细胞ATP含量的影响。

Fig.6 Effects of interfering with BPGM on glycolysis and the content of IMP and ATP in myoblasts A.Expression of upstream and downstream genes in the G/G pathway after interfering with the BPGM gene; B.The effect of interfering with BPGM on IMP content in adult myocytes;C.The effect of interfering with BPGM on ATP content in adult myocytes.

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BPGM下调对静原鸡成肌细胞生长发育的影响

The Effect of Down-regulation of BPGM on the Growth and Development of Myogenic Cells in Jingyuan Chickens

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