山羊<i>FGF9</i>基因克隆及其在成肌细胞分化中的表达模式研究

doi:10.7668/hbnxb.201751384

摘要/Abstract

摘要： 旨在克隆山羊FGF9基因序列并对其进行生物信息学分析，阐明FGF9基因组织表达特性及其在成肌细胞分化过程中的表达差异。试验动物为简州大耳羊，利用RT-PCR技术克隆FGF9基因序列，再用实时荧光定量PCR（qRT-PCR）技术检测FGF9在山羊各组织中的表达特性及其在成肌细胞不同分化阶段的表达情况。结果显示，克隆得到山羊FGF9基因序列818 bp，其中ORF区627 bp，编码208个氨基酸，其CDS区核苷酸序列与牛和绵羊有99%的同源性。FGF9蛋白具有1个跨膜结构域和1个FGF家族同源性结构域，为不稳定亲水蛋白。FGF9基因在山羊各组织中均有表达，在肾脏中表达水平最高，极显著高于其他组织（P<0.01）。FGF9基因在诱导分化第2天表达水平显著高于分化前（P<0.05），且在第4天达到极显著水平（P<0.01）。推测其可作为调控山羊成肌细胞分化的候选基因。为进一步探究FGF9基因在山羊肌肉生长中的作用提供理论依据。

关键词: 山羊, FGF9, 克隆, 生物信息学分析, 表达特性, 成肌细胞

Abstract: The aim of this study was to clone the goat FGF9 gene sequence and perform bioinformatics analysis, so as to elucidate the tissue expression characteristics of FGF9 and its differential expression during myoblast differentiation. The experimental animal of this study was the Jianzhou Big-eared sheep. The FGF9 gene sequence was cloned by RT-PCR. Real-time quantitative PCR (qRT-PCR) was performed to detect the expression of FGF9 in different tissues of goat and at different stages of myoblast differentiation. The results showed that the sequence of goat FGF9 gene was 818 bp, with an ORF region of 627 bp, encoding 208 amino acids. The nucleotide sequence of the CDS region was 99% homologous to Bostaurus and Ovisaries. The FGF9 protein had a transmembrane domain and a FGF family homology domain, which was an unstable hydrophilic protein. The FGF9 gene could express in all tissues of goats, and the expression level in the kidney was the highest, which was significantly higher than in other tissues (P<0.01). The expression level of FGF9 gene in the 2nd day of induced differentiation was significantly higher than that before differentiation (P<0.05), and reached a very significant level in the 4th day (P<0.01). This study provided the important data for further exploring the role of FGF9 gene in goat muscle growth.

Key words: Goat, FGF9, Clone, Bioinformatics analysis, Expression characteristics, Myoblast

中图分类号:

S826

黄凯, 林亚秋, 朱江江, 马洁琼, 王永. 山羊FGF9基因克隆及其在成肌细胞分化中的表达模式研究[J]. 华北农学报, 2019, 34(3): 232-238. doi: 10.7668/hbnxb.201751384.

HUANG Kai, LIN Yaqiu, ZHU Jiangjiang, MA Jieqiong, WANG Yong. Cloning of Goat FGF9 Gene and Its Expression Pattern in Myoblast Differentiation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 232-238. doi: 10.7668/hbnxb.201751384.

http://www.hbnxb.net/CN/Y2019/V34/I3/232

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山羊FGF9基因克隆及其在成肌细胞分化中的表达模式研究

Cloning of Goat FGF9 Gene and Its Expression Pattern in Myoblast Differentiation

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山羊FGF9基因克隆及其在成肌细胞分化中的表达模式研究

Cloning of Goat FGF9 Gene and Its Expression Pattern in Myoblast Differentiation

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