摘要： 为选育抗旱性强、耗水少、水分利用效率高的品种。以22个玉米自交系为材料,采取正常灌溉和适度水分胁迫处理,对自交系叶片水分利用效率(WUE)、光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、胞间CO₂浓度(Ci)、相对叶绿素值(SPAD)和荧光动力学参数进行研究。结果表明,不同自交系WUE差异显著,而且对水分的敏感程度也存在显著差异;适度水分胁迫使Gs、Pn、Tr都有所下降,但各材料的下降幅度不同,其中Tr的下降幅度要大于Pn下降的幅度,使得WUE比正常灌水处理下有所升高;自交系WUE与Pn、最大荧光(Fm)、可变荧光(Fv)、PSⅡ原初光能转换效率(Fv/Fm)及PSⅡ潜在活性(Fv/Fo)达到显著相关。

关键词: 玉米, 自交系, 叶片水分利用效率, 生理变化

Abstract: In order to choose the appropriate receptor of photo-thermo-sensitive male sterile wheat for bombardment,and optimize the Culture condition of immature embryos,We used immature embryos of 3 wheat lines,including photo-thermo-sensitive male sterile line BS210,BS366 and normal cultivar Jing 411,Carbon source,screening concentration and genotype were screened respectively.The study showed that the frequency of embryos callus formation is not significantly different between sucrose and maltose as the carbon source.While adding maltose in different mediums,the frequency of callus differentiation was higher than the medium adding sucrose.During screening callus that the bar gene had bombarded in,the optimal concentration of herbicides biolaphos was 2 mg/L.The seeding rate of three material was significantly different.The seeding rate of BS210 was higher than other plants,which is the best recipient for transformation.The research sets the foundation for the improvement of photo-thermo-sensitive male sterile wheat.

Key words: Maize, Inbred line, Water use efficiency, Physiological changes

中图分类号: 

• S513．01