摘要： 为了选择适宜的基因枪转化光温敏雄性不育小麦的受体,并对幼胚组织培养条件进行优化,以小麦光温敏雄性不育系BS210、BS366以及常规品种京411的幼胚为材料,分别对碳源、除草剂浓度和基因型进行筛选。结果表明,在以蔗糖和麦芽糖为碳源的诱导培养基上,幼胚的出愈率差异不显著;在以蔗糖和麦芽糖为碳源的分化培养基上,愈伤组织的分化率差异显著,其中添加麦芽糖的培养基上分化率较高;在对轰击过bar基因的幼胚愈伤组织进行筛选时,除草剂Biolaphos的最适浓度为2 mg/L。3个受体材料离体培养过程中出苗率差异显著,其中BS210出苗率高,是较优良的转化受体,为今后利用转基因技术改良光温敏雄性不育小麦奠定了基础。
关键词:
小麦幼胚,
光温敏雄性不育,
碳源,
除草剂浓度,
基因型
Abstract: Lilium brownii F.E.Brown ex Miellez,Brown lily,belongs to Lilium of Liliaeeae in planttaxonorny,is a endemic species and one of commonlly-used cross breeding parents of Lilium in China.L.brownii F.E.Brown ex Miellez var.virdulum Baker,Greenish lily,a variety of Lilium brownii,is also a well known medicinal and edible plant.Wild germplasm resources are the basic materials for genetic research and plant breeding.Howerever,due to Lilium brownii is scattered in the wild and its collection is generally only in flowering phase,it is a great difficulty for its scientific research.In the year before,researches on Lilium brownii especially molecular biology researches are still lacking in Guangdong province.In this study,total 199 Lilium brownii samples of 7 natural populations collected from representative mountain areas in Guangdong China were analyzed by RAPD markers,so as to provide good foundation for further natural germplasm resources and related studies of Lilium brownii populations,and provide good evidence of the discrimination of Brown lily and Greenish lily at molecular level.DNA from all samples was extracted from leaves and scales,which amplified clear,bright and steady RAPD bands.20 primers were screened from 273 random primers,and a total 433 DNA bands were amplified,430(99.3%) of which were polymorphic,the percentage of polymorphic bands(PPB) at the population level was 60.67% on average.The unweighted pair group method arithmetic average(UPGMA) clustery analysis of the RAPD data from 199 samples showed that all Brown lily samples and Greenish lily samples in the same population separated obviously at the value of 0.68 of genetic similarity coefficient,which was a good evidence of the discrimination of Brown lily and Greenish lily at molecular level.
Key words:
Wheat immature embryos,
Photo-thermo-sensitive male sterility,
Carbon source,
Concentration of herbicide,
Genotype
中图分类号:
王灵云, 张立平, 赵昌平, 张风廷, 樊建青, 苑少华, 郭玉平. 基因枪转化光温敏雄性不育小麦受体的选择与培养条件优化[J]. 华北农学报, 2011, 26(4): 126-129. doi: 10.7668/hbnxb.2011.04.022.
WANG Ling-yun, ZHANG Li-ping, ZHAO Chang-ping, ZHANG Feng-ting, FAN Jian-qing, YUAN Shao-hua, GUO Yu-ping. Random Amplified Polymorphic DNA (RAPD) Analysis of Natural Lilium brownii from Guangdong,China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 126-129. doi: 10.7668/hbnxb.2011.04.022.