低温处理对水稻正反交F<sub>1</sub>雄配子基因型频率的影响

doi:10.3969/j.issn.1000-7091.2012.05.020

摘要/Abstract

摘要： 利用来自云南海拔2 670 m高寒稻区的地方粳稻品种小麻谷与具有籼稻细胞质背景的籼粳交品系南34进行正反交产生F1,用经14℃和17℃低温离体处理的正反交F1的稻穗与育性稳定的1个滇Ⅰ型细胞质不育系授粉获得3个测交群体。对F1的花粉育性和所获得测交群体的PCR位点进行分析,发现温度及细胞质背景都对F1产生的雄配子基因型发生了选择,导致雄配子基因型的遗传比例偏离孟德尔规律。对这一问题的研究有助于探讨温度对水稻遗传分化的影响,还可探讨通过对雄配子基因型的差异选择提高定向育种效率的育种方法,将有助于探讨全球气候变化对水稻及其他作物的遗传变异将产生的影响。

关键词: 基因型频率, 水稻, 雄配子选择, 低温处理, 细胞质

Abstract: The F1 plants were produced by reciprocal cross between a cv.Xiaomagu,a cold tolerant japonica landrace collected at 2 670 m altitde with cv.Nan 34,which was derived from mult-crosses between indica and japonica.Three populations were developed by pollinating a cytoplasmic male sterile(CMS) line of Dian typeⅠwith the panicles of the F1S treated by 14℃and 17℃ temperatmes in vitro.The results showed that the temperature and the cytoplasmic background led to the selection of F1 androgamete genotype,which result in the deviation of male gamete genotypes proportion from Mendelian fashion,after the pollen fertility of F1′s and PCR locus of its test-cross population have been analyzed.Studies on the issue would make benefit to understanding rice genetic differentiation resulted from temperature variation,and benefit to exploit approach of direction breeding based on genotype selection of male gametes,and also benefit to understanding of genetic variation brought by global climate change.

Key words: Genotype frequency, Rice(Oryza sativa L.) Androgamete selection, Treatment of low temperature, Cytoplasm

中图分类号:

S511

张云, 谭学林, 文建成, 谭亚玲. 低温处理对水稻正反交F₁雄配子基因型频率的影响[J]. 华北农学报, 2012, 27(5): 104-109. doi: 10.3969/j.issn.1000-7091.2012.05.020.

ZHANG Yun1, TAN Xue-lin, WEN Jian-cheng, TAN Ya-ling. Effects of Low Temperature Treatment on Androgamete Genotype Frequencies of Rice Reciprocal Cross F_1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 104-109. doi: 10.3969/j.issn.1000-7091.2012.05.020.

http://www.hbnxb.net/CN/Y2012/V27/I5/104

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低温处理对水稻正反交F₁雄配子基因型频率的影响

Effects of Low Temperature Treatment on Androgamete Genotype Frequencies of Rice Reciprocal Cross F_1

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低温处理对水稻正反交F1雄配子基因型频率的影响

Effects of Low Temperature Treatment on Androgamete Genotype Frequencies of Rice Reciprocal Cross F_1

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低温处理对水稻正反交F₁雄配子基因型频率的影响