摘要： 本研究选择了中国广东省7个有代表性的山区作为野百合(Lilium brownii F.E.Brown ex Miellez)天然居群的采样点,对采集到的199份样品进行了随机扩增多态性DNA(RAPD)分析,以建立野百合天然居群RAPD分析方法体系以便后续研究,并了解广东省野百合天然居群多态性情况和居群内外个体及野百合及变种百合样品的聚类情况。以新鲜叶片、硅胶干燥叶片及鳞片为材料分别提取DNA,从273条10聚寡核苷酸随机引物中筛选出20条随机引物,对7个居群共199个野百合及百合样品进行RAPD扩增。共检测到433条RAPD谱带,其中多态性条带为430条,所有个体的多态性条带百分率(PPB)为99.31%,各居群的平均多态位点比率为60.67%。通过对199个个体的RAPD数据的UPGMA聚类分析,获得了聚类图。199个样品用三种方法都成功提取出质量较高的DNA,并能扩增出清晰、明亮、稳定的RAPD条带。在聚类图遗传相似系数为0.68处发现同一居群内的所有百合个体和野百合个体在聚类图中表现了明显的分离,这是原变种野百合及变种百合的区别在分子水平上很好的反映。

关键词: Lilium brownii, 野百合, 百合, RAPD, 聚类分析

Abstract: Pathogenicity determination and Pot2rep-PCR techniques were used to analyze the diversity of Magnaporthe.grisea from Jiangsu in 2007 and 2008,respectively.They were divided into 10 Physiological race and 14 lineages,in which ZG1 and JSL5 are dominant.The results showed that the rice blast fungus are rich in diversity in Jiangsu,but when compared with the group structure of 1999-2002,it tends to be more simple;By analyzing temporal and spatial distribution of M.grisea,we found that Population structure of M.grisea varied significantly between years and diferent rice cultivation areas of Jiangsu;By combining pathotypes and genetic lineages analysis,no obvious corresponding relationship was found between them.There is a certain similarity in region variations between pathotypes and genetic lineages,which indicated that there is something in common between pathotypes and DNA polymorphism examination at the macro-level,therefore,to a certain extent,they can reflect the diversity of Magnaporthe grisea all.

Key words: Lilium brownii, Brown lily, Greenish lily, RAPD, Cluster Analysis

中图分类号: 

• Q751