摘要： 为研究蓝狐GH基因多态性及其与生长性状的关系，为蓝狐的选种及选育提供一定的理论依据。选取体质量、体长具有明显差异的芬兰狐和地产狐构建DNA池，采用PCR产物直接测序法对GH基因的全长进行单核苷酸多态性（Single nucleotide polymorphism，SNPs）位点的检测，并将发现的SNPs位点与生长性状进行关联分析。结果检测到2个SNPs，GH基因A306G和C1144T位点在芬兰狐和地产狐群体中存在多态性。遗传多样性分析表明，2个位点在芬兰狐中多态信息含量为0.057 9，属于低度多态位点，地产狐多态信息含量为0.573 1，属于高度多态位点。2个SNPs位点的遗传多样性参数、基因型频率和基因频率在同一群体中高度一致，说明这2个SNPs位点之间完全连锁，形成了AACC、ABCD和BBDD 3种单倍型。方差分析表明，芬兰狐和地产狐多态位点的基因型频率差异极显著（P <0.01）。关联分析表明，公狐AACC基因型个体体质量显著高于ABCD基因型（P <0.05），但与体长差异不显著（P >0.05）；母狐AACC基因型个体体质量极显著高于ABCD基因型个体（P <0.01）且体长显著高于ABCD基因型个体（P <0.05）。结果提示：2个SNPs与其生长性状可能相关。

关键词: 芬兰狐, 地产狐, DNA池, 单核苷酸多态性, 生长激素基因, 基因型

Abstract: The research was to study the relationship of the polymorphism of GH gene on body measurement traits in blue fox gene,providing some evidence for the selection and breeding in blue fox gene. Finland blue fox and local blue fox with significant differences in body weight and body length were chosen for testing subjects,DNA pooling was constructed for each species. The technology of direct sequencing of PCR products was used to detected the single nucleotide polymorphism of fox growth hormone gene. And correlation analysis between GH polymorphisms and growth traits was carried out. Results showed that two SNPs,A306G and C1144T were found within finland blue fox and local blue fox.The genetic structure analysis demonstrated that the two SNPs loci in the finland blue fox were low polymorphism loci (0.057 9) and local blue fox were high polymorphism loci (0.573 1). The two SNPs loci were the same in the genetic diversity parameters,the frequencies of alleles and genotypes,thus had complete genetic linkage,with the formation of AACC,ABCD and BBDD. The analysis of variance showed that the gene frequency difference among finland blue fox and local blue reached at the extremely significant level(P <0.01). Moreover,we analyzed the correlation between the genotypes of SNPs loci and the growth traits of the two groups.The male fox body weight of AACC genotype were significantly higher than those of ABCD genotype(P <0.05),but there was no significant difference in body length (P >0.05).The female fox body weight of AACC genotype were extremely significantly higher (P <0.01) and body length significantly higher than those of ABCD genotype(P <0.05). These results indicated that the two SNPs might be related to the growth performance.

Key words: Finland blue fox, Local blue fox, DNA pooling, Single nucleotide polymorphisms (SNPs), Growth hormone gene, Genotype

中图分类号: 

• S865.2