摘要： 为了研究胰岛素样生长因子1受体（IGF-1R）基因外显子2和外显子11的单核苷酸多态性，分析该基因变异与水貂生长性状的关联性，以红眼白水貂、金州黑水貂和咖啡水貂共计235个样本的血液基因组DNA为模板，采用PCR产物克隆和Sanger测序技术，筛查IGF-1R基因SNP位点，将差异位点与不同种群的水貂生长性状进行关联分析。结果表明，红眼白、金州黑水貂的外显子2和外显子11上检测到2个SNPs：c.207G>A和c.1782G>A；咖啡水貂在外显子2上检测到2个SNPs：c.207G>A和c.218T>A，在外显子11检测到1个SNPs：c.1782G>A。c.207G>A和c.1782G>A都属于无义突变。c.218T>A位点属于有义突变，氨基酸发生改变，脯氨酸变为精氨酸。关联分析表明：c.207G>A与金州黑水貂和咖啡水貂体长性状显著相关（P<0.05），其中GG基因型个体为水貂体长的优势基因型；c.1782G>A与咖啡水貂的体长和体质量显著相关（P<0.05），且与咖啡水貂的体质量呈现极显著差异（P<0.01），GA基因型作为咖啡水貂的优势基因型，其杂合GA基因型个体的体长、体质量都显著高于纯合GG基因型和纯合AA基因型个体。合并基因型进行关联分析发现：金州黑水貂合并基因型GGGA个体和GGAA个体的体长与GAGA个体体长差异极显著（P<0.01），基因型GGGA个体和GGAA个体的体长高于GAGA个体体长；金州黑水貂合并基因型GGGA个体体质量与GAGA个体差异显著（P<0.05），GGGA个体体质量高于GAGA基因型个体。试验可推断得出c.207G>A位点GG基因型是水貂体长的优势基因型；合并基因型GGGA和GGAA可能是影响金州黑水貂体长体质量的有利基因型，认为IGF-1R基因可能是影响水貂生长性状的候选基因。

关键词: 水貂, IGF-1R基因, 单核苷酸多态性, 生长性状, 关联分析

Abstract: The aim of the paper was to study the single nucleotide polymorphism of Insulin-like growth factor 1 receptor(IGF-1R)gene and its relationship with the growth trait of mink. A total of 235 blood samples from 3 mink varieties(Red-eye white, Jinzhou black and Coffee)were taken as the DNA templates to screen the SNPs by PCR product cloning and Sanger sequencing technology, and the relationships between the mutation sites of IGF-1R gene and the growth traits of different populations were analyzed. The results showed that two SNPs were detected in exon 2 and exon 11 of red-eye white mink and Jinzhou black mink:c.207G > A and c.1782G > A; Two SNPs were detected in exon 2 of coffee mink:c.207G > A and c.218T > A, and one SNP was detected in exon 11:c.1782G > A. Both c.207G > A and c.1782G > A belonged to nonsense mutations, and c.218T>A site belonged to a sense mutation, the later mutation being an amino acid change from proline to arginine. The association analysis showed that the c.207G>A had significant association with the body length of Jinzhou black mink and coffee mink(P<0.05), and GA genotype might be a favorable genotype affecting the body length of mink;while c.1782G>A had significant association with the body length of coffee mink and had extremely significant assosiation with the body weight of coffee mink(P<0.01). The heterozygous genotype(GA)was the dominant genotype, and its body length and body weight were significantly higher than GG genotype and AA genotype individuals. The association analysis of the combined genotypes showed that the different genotypes of Jinzhou black mink were significantly correlated with the body length and body weight. The body length of the combined genotype GGGA individuals and GGAA individuals were extremely significantly higher than other genotypes (P<0.01); The body weight of the combined genotype GGGA individuals were significantly higher than GAGA genotypes individuals (P<0.05); It was preliminarily concluded that the GGGA and GGAA genotypes might be a favorable genotype affecting the body weight of Jinzhou black mink. The above results indicated that IGF-1R gene might be the candidate gene affecting the growth trait of mink.

Key words: Mink, IGF-1R gene, Single nucleotide polymorphism, Growth traits, Association analysis

中图分类号: 

• S865.2

• Q78