槟榔江水牛<i>BMP4</i>基因分离鉴定、组织表达及生物信息学分析

doi:10.7668/hbnxb.201751553

摘要/Abstract

摘要： BMP4基因在小鼠、人类及普通牛的胚胎发育、卵泡发生发育和精子形成过程中发挥重要作用，但在水牛上的研究尚未见报道。旨在探究水牛BMP4基因序列及组织表达特征，以揭示其功能。根据NCBI数据库普通牛BMP4序列设计引物，分离鉴定了槟榔江水牛BMP4编码区全长序列（KF312880）。随后采用生物学软件和半定量RT-PCR技术对水牛BMP4进行了分子特征和组织表达差异分析。水牛BMP4编码区长度与其他牛科物种的相同，全长1 230 bp，编码409个氨基酸。水牛BMP4蛋白为亲水性蛋白，含有1个信号肽序列（AA 1-24）和6种功能活性位点，无跨膜结构域，属胞外分泌蛋白，具有TGF-beta前导肽（AA 39-276）和TGF-beta超家族（AA 309-409）结构域。在BMP4氨基酸序列上，水牛与普通牛、野牦牛、人、马等10个物种的一致性≥ 97%。在所检测的11个组织中，BMP4仅在水牛子宫、卵巢和睾丸中表达，且在睾丸组织中表达水平最高。揭示水牛与普通牛的BMP4结构相似，功能相近，推测BMP4基因在水牛胚胎发育和生殖细胞成熟过程中发挥作用。

关键词: 槟榔江水牛, BMP4基因, 基因分离鉴定, 组织表达谱, 生物信息学分析

Abstract: BMP4 gene plays a vital role in the embryonic development, follicular development and spermatogenesis of human, mouse and cattle, but the research on buffalo has not been reported yet. The purpose of this study was to explore the sequence and tissue expression characteristics of buffalo BMP4 gene in order to reveal its function. The full length of buffalo BMP4 coding region was isolated and identified(KF312880)by the primers designed based on the cattle BMP4 sequence in NCBI database. Then, the molecular characteristics and tissue expression difference of buffalo BMP4 were analyzed using bioinformatics software and semi-RT-PCR technique. The total length of buffalo BMP4 coding region was 1 230 bp, which has the same length with other bovine species, and encoded a peptide of 409 amino acid residues. The buffalo BMP4 protein was a hydrophilic protein without transmembrane domain, containing a signal peptide sequence(AA 1-24)and six kinds of functional active sites. And this protein belonged to extracellular secreted protein, which had a domain of TGF-beta precursor peptide(TGF-beta propeptide)(AA 39-276)and a domain of TGF-beta superfamily(AA 309-409). The amino acid sequence of buffalo BMP4 shared over ≥ 97% homology with that of 10 other species such as wild yak, common cattle, human, horse, etc. Among the 11 buffalo tissues assayed, it was found that the BMP4 expressed only in uterus, ovary and testis, with the highest expression level in testis.The polypeptide encoded by buffalo BMP4 was similar to cattle in structure and function. The results indicated that buffalo BMP4 might play a role in buffalo embryo development and germ cell maturation.

Key words: Binlangjiang buffalo, BMP4 gene, Gene isolation and identification, Tissue expression profile, Bioinformatics analysis

中图分类号:

S823.83

郭文博, 哈福, 江明星, 张自芳, 钱林东, 保志鹏, 苗永旺. 槟榔江水牛BMP4基因分离鉴定、组织表达及生物信息学分析[J]. 华北农学报, 2019, 34(4): 223-230. doi: 10.7668/hbnxb.201751553.

GUO Wenbo, HA Fu, JIANG Mingxing, ZHANG Zifang, QIAN Lindong, BAO Zhipeng, MIAO Yongwang. Isolation, Identification, Tissue Expression and Bioinformatics Analysis of BMP4 Gene from Binlangjiang Buffalo[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 223-230. doi: 10.7668/hbnxb.201751553.

http://www.hbnxb.net/CN/Y2019/V34/I4/223

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槟榔江水牛BMP4基因分离鉴定、组织表达及生物信息学分析

Isolation, Identification, Tissue Expression and Bioinformatics Analysis of BMP4 Gene from Binlangjiang Buffalo

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槟榔江水牛BMP4基因分离鉴定、组织表达及生物信息学分析

Isolation, Identification, Tissue Expression and Bioinformatics Analysis of BMP4 Gene from Binlangjiang Buffalo

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