摘要： 为探讨腐烂病胁迫下核桃枝条生化代谢和树体抗病性之间相互作用关系，随机选取健康、轻度、中度和重度4种病害程度植株各7株，然后运用线性回归分析各枝条POD、Pro、PAL活性及核桃长势（核桃基茎）与病害程度（病斑面积）关系。结果表明：3种抗氧化酶活性的变化规律具有一定差异，其中PAL活性最高值分别出现在轻度病害植株叶片和中度病害枝条，而POD活性的极大值则出现在健康植株叶片和中度病害枝条，叶片中Pro活性与POD显示相似规律，均显著高于其他病害样品活性（P<0.05）。另外枝条Pro活性具有双峰特征即侵染初期其活性快速升高，病害程度进一步加剧其活性反而显著降低，随着病害发展其活性又明显增加；PAL活性和Pro活性极小值出现在病害侵染前期，POD活性极小值则出现在病害发展末期。相关分析显示，病害程度受POD活性和核桃长势（核桃基茎）极显著负影响，相关性分别达到-0.76（P<0.01），-0.85（P<0.01）；病害程度受Pro极显著影响，相关性为0.52（P<0.01）。由此可见，腐烂病侵染过程中，增强核桃长势即增加核桃基茎对腐烂病侵染过程有极强抑制作用，另外也表明，核桃植株体内POD活性增加，有利于增强核桃抵抗腐烂病的抗性。

关键词: 核桃腐烂病, POD活性, Pro活性, 核桃基茎, 病斑面积, 多元回归分析

Abstract: To explore the interaction between the biochemical metabolism of walnut branches and the disease resistance of trees under the stress of Jugladis canker.Randomly selected from health, mild, moderate and severe degree of 4 kinds of disease plant each 7 plants, then use Duncan (P<0.05) and single factor analysis of variance blade 3 branches in 3 different antioxidant enzyme activity, multivariate linear regression analysis by using the PAL (phenylalanine ammonia-lyase) activity, POD (peroxidase) activity and Pro (proline) of branches,and walnut base stem relates to the degree of disease (disease spot size).Results showed that the change of the three kinds of antioxidant enzyme activity had certain difference, the PAL activity peak appears in the mild disease leaves and moderate disease branches, while the POD activity of maximum health plant leaves and branches moderate disease, leaf Pro and POD activity of showed similar rule, samples were significantly higher than other disease activity (P<0.05).In addition, the activity of branch Pro had a bimodal characteristic, that is, its activity increased rapidly at the initial stage of infection, while its activity decreased significantly if the disease was further aggravated, and increased significantly with the development of disease.The minima PAL and Pro activity appeared in the early stage of disease infection, and POD activity minima appeared in the late stage of disease development.Correlation analysis showed that POD activity and walnut growth (walnut base stem) had significant negative effects on disease degree, and the correlation reached -0.76 (P<0.01) and -0.85 (P<0.01), respectively.The disease degree was significantly affected by Pro, and the correlation was 0.52 (P<0.01).Therefore, during the infection process of rot disease, increasing the growth of walnut, that is, increasing the base and stem of walnut, has a strong inhibitory effect on the infection process of rot disease. In addition, it also showed that the increased POD activity in walnut plants is conducive to strengthening the resistance of walnut to rot disease.

Key words: Jugladis canker, POD activity, Proline activity, Stems of walnut, Canker area, Multiple linear regression analysis

中图分类号: 

• S664.1