低温对不同基因型黄瓜幼苗生长及生理特性的影响

doi:10.7668/hbnxb.2013.06.025

摘要/Abstract

摘要： 人工低温条件下比较了EC1、北京截头和SWCC12 3种黄瓜苗期在形态、生理及PSⅡ对低温响应的差异。结果表明:低温胁迫均使3种黄瓜产生冷害,EC1植株脱水最轻,SWCC12冷害最严重;3种黄瓜的电渗率和丙二醛(MDA)因低温处理而增加,变化最小的是EC1,最大的是SWCC12;低温处理提高了3种黄瓜中的超氧化物歧化酶(SOD)活性,尤其是EC1和北京截头的SOD活性反应更为高效和快速,EC1中过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)呈先增加后降低趋势,但始终高于处理前的,而北京截头中分别先降低后升高及变化不明显,SWCC12中主要变化趋势为增加,EC1体内的过氧化氢酶(CAT)在低温处理2 d时活性最高,之后下降至原来水平,北京截头和SWCC12中变化不大;3种黄瓜体内可溶性蛋白在胁迫处理时都呈先升高后降低趋势;EC1和北京截头中的可溶性糖也表现出先升高后降低现象,但都显著高于处理前,而SWCC12中可溶性糖呈显著下降趋势;3个基因型黄瓜内叶绿素含量和类胡萝卜素含量随胁迫时间先增加后降低,EC1中维持高含量时间长,下降幅度最小,SWCC12下降幅度最大;低温均影响了3份黄瓜的PSⅡ、Fv/Fm、Yield和ETR,下降幅度最大的是SWCC12、最小的是北京截头。

关键词: 黄瓜, 基因型, 低温胁迫, 抗氧化酶, PSⅡ

Abstract: The responses of seedling growth, physiological properties and PSⅡto low temperature among three genotype cucumbers were comparatively studied.The results showed that:Chilling injury of three genotypes pro-duced under low temperature stress, the dehydrate and chilling injury of EC 1 was the most light, and SWCC12 showed the serious.The contents of Malondialdehyde ( MDA) and electrolyte leakage in three cucumbers increased under cold treatment,the increase extent of two indices in EC1 was minimum,and in SWCC12 was maximal.Super-oxide dismutase ( SOD) in three cucumbers increased during treatment, especially in EC1 and Beijingjietou SOD were induced rapidly and efficiently.The change of peroxidase ( POD) and ascorbate peroxidase ( APX) activity in EC1 rose first and then fell, but were higher than pretreatment, and the contents of two enzymes in Beijingjietou first decreased the increased and had no significant change.While the activity of POD and APX in SWCC 12 increased under low temperature stress.Catalase(CAT) activity in EC1 could be activated by low temperature,reaching the peak in 2 d and then dropped to the former level, that in Beijingjietou and SWCC 12 had not changed much.The content of soluble protein increased before decreasing in three genotypes.And the change of soluble sugar in EC 1 and Beijingjietou as the same as that protein, and were higher than pretreatment, but in SWCC12 declined during stress.The contents of chlorophyll and carotenoid in three genotypes first increased and then decreased under low temperature.The high contents of two pigments in EC1 maintained longer time, declined slowly, while that in SWCC12 was contrary.Low temperature hurt the PSⅡ of cucumbers,the Fv/Fm,Yield and ETR in SWCC12 di-clined most seriously, while in Beijngjietou most slightly.

Key words: CucumbeR, Genotype, Low tempeRatuRe stRess, Antioxidant enzyme, PhotosystemⅡ

中图分类号:

S642.2

苗永美, 宁宇, 魏庆镇, 陈劲枫. 低温对不同基因型黄瓜幼苗生长及生理特性的影响[J]. 华北农学报, 2013, 28(6): 140-146. doi: 10.7668/hbnxb.2013.06.025.

MIAO Yong-mei, NING Yu, WEI Qing-zhen, CHEN Jin-feng. Effects of Low TempeRatuRe on GRowth and Physiological ChaRacteRistics of DiffeRent CucumbeRs Genotypes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 140-146. doi: 10.7668/hbnxb.2013.06.025.

http://www.hbnxb.net/CN/Y2013/V28/I6/140

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