摘要： 为了探明硫化氢调控植物响应盐胁迫的机理，以黄瓜幼苗为试材，通过浇灌Hoagland营养液（CK）、200 mmol/L NaCl（T）和200 mmol/L NaCl+15 μmol/L NaHS（H₂S供体试剂，S）处理，利用Illumina HiSeq高通量测序对黄瓜根系进行转录组测序及差异表达基因分析。结果发现，9个样本获得高质量序列共365.35 Mb，与参考基因组进行比对，比例为86.27%~88.64%的序列总数为315.74 Mb。差异表达基因分析显示：T组较CK组上调基因有1 168个，下调基因有1 076个；S组较T组上调基因有435个，下调基因有218个。GO富集分析显示：差异表达基因主要富集在分子功能大类中的结合和催化活性类，生物过程大类中的代谢过程、细胞过程和单一生物过程类及细胞组分大类中的膜和膜组分类中。KEGG富集分析将3个比较组的差异表达基因分别归为139，75，127个代谢通路，T-S比较组的差异表达基因主要富集在植物激素信号转导、内质网中的蛋白质加工和光合作用通路中。筛选10个可能与H₂S调控黄瓜响应盐胁迫相关的基因：CSC1样蛋白ERD4基因、NADH型硝酸还原酶样基因、蛋白质TIFY 10B样基因、丙二烯氧化物环化酶基因、BTB/POZ和TAZ结构域蛋白1样基因、麦冬蛋白-3样基因、丝裂原激活蛋白激酶激酶3样基因、转录因子bHLH18样基因、IAA-氨基酸水解酶ILR1样4基因和钾通道AKT1基因。研究H₂S调控黄瓜响应盐胁迫的机理，为提高植物耐盐性提供了理论数据和参考依据。

关键词: 黄瓜, H₂S, NaCl胁迫, GO分析, KEGG分析

Abstract: In order to verify the mechanism of hydrogen sulfide to regulate the plant response to salt stress, cucumber seedlings were used as materials and treated with Hoagland(CK), 200 mmol/L NaCl(T) and 200 mmol/L NaCl+15 mmol/L NaHS (the donor of H₂S, S). The expression of transcriptome genes of cucumber roots were studied by using Illumina HiSeq high-throughput sequencing technology. A total of 365.35 Mb of Clean reads were obtained from 9 samples. Mapped reads was 315.74 Mb, and the percentage was 86.27%-88.64%. Analysis of differentially expressed genes revealed that T group had 1 168 genes up-regulated and 1 076 genes down-regulated compared with CK group, and S group had 435 genes up-regulated and 218 genes down-regulated compared with T group. The Gene Ontology (GO) enrichment analysis revealed that the differentially expressed genes were mainly concentrated in binding and catalytic activity in molecular functional classes; in biological process classes, they were mainly enriched in metabolic processes and cellular processes and single-organism process; Among cell components, the membrane and membrane part classes were mainly concentrated. KEGG enrichment analysis of the differentially expressed genes could be divided into 139, 75 and 127 pathway of 3 comparison groups, respectively. The differentially expressed genes in S group compared to the T group were mainly abundant during plant hormone signal transduction, protein processing in endoplasmic reticulum and photosynthesis pathway. Screening of 10 genes that might be related to H₂S regulating cucumber response to salt stress:CSC1-like protein ERD4, nitrate reductase (NADH)-like, protein TIFY 10B-like, allene oxide cyclase, BTB/POZ and TAZ domain-containing protein 1-like, tricalbin-3-like, mitogen-activated protein kinase kinase 3-like, transcription factor bHLH18-like, IAA-amino acid hydrolase ILR1-like 4 and potassium channel AKT1. Studying the mechanism of H₂S regulating cucumber response to salt stress provides theoretical data and reference basis for improving plant salt tolerance.

Key words: Cucumber, H₂S, NaCl stress, GO analysis, KEGG analysis

中图分类号: 

• Q78

• S642.01