华北农学报 ›› 2013, Vol. 28 ›› Issue (6): 136-139. doi: 10.7668/hbnxb.2013.06.024

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五种短体线虫DNA条形码鉴定方法

魏亚东1, 容万韬1,2, 赵立荣3, 王金成1, 黄国明1, 郭京泽1, 孙建华2   

  1. 1 天津出入境检验检疫局动植物与食品检测中心,天津,300461;
    2 天津师范大学, 天津 300387;
    3 广东出入境检验检疫局,广东 广州,510623
  • 收稿日期:2013-07-19 出版日期:2013-12-28
  • 通讯作者: 王金成(1978-),男,山东安丘人,高级农艺师,主要从事植物线虫检疫研究。
  • 作者简介:魏亚东(1967-),男,河北唐山人,高级农艺师,主要从事植物检疫及相关研究。
  • 基金资助:
    国家质检总局项目(2011IK176;2011IK177);国家科技支撑计划项目(2012BAK11B03)

Identification of 5 PRatylenchus Species Utilizing DNA BaRcoding Method

WEI Ya-dong1, RONG Wan-tao1,2, ZHAO Li-rong3, WANG Jin-cheng1, HUANG Guo-ming1, GUO Jing-ze1, SUN Jian-hua2   

  1. 1 Animal Plant and Food Stuffs Inspection Center, Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300461, China;
    2 Tianjin Normal University, Tianjin 300387, China;
    3 Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China
  • Received:2013-07-19 Published:2013-12-28

摘要: 以穿刺短体线虫、咖啡短体线虫、伤残短体线虫、斯克里布纳短体线虫和落选短体线虫5种短体线虫的18个种群为试验材料,将其测序获得的ITS序列与GenBank中已提交的短体线虫序列进行了Blast比对和系统发育分析,以验证核糖体ITS序列作为DNA条形码的可行性。结果显示,核糖体ITS序列作为DNA条形码可以很好的用于鉴别以上5种短体线虫。

关键词: 短体线虫, ITS, 系统发育, DNA条形码

Abstract: The root lesion nematodes,Pratylenchus Flipjev,1936,are among the widespread and seriously de-structive plant endoparasites around the world.Due to the instability of morphological characteristics, it was very dif-ficult to identify this kind of nematodes just by their morphology.In this study, 18 populations of the following 5 Pra-tylenchus species,i.e.Pratylenchus penetrans,P.coffeae,P.vulnus,P.scribneri and P.neglectus, were used as ex-periment materials, and their ribosomal ITS sequences were obtained to conduct blast alignment and phylogenetic a -nalysis with other ribosomal ITS sequences of Pratylenchus species submitted in GenBank in order to verify the fea-sibility of ITS regions as DNA barcode.The results showed that DNA barcoding approach using ribosomal ITS re-gions as target fragment could be readily used for identification of the above-mentioned 5 Pratylenchus species.

Key words: PRatylenchus, ITS, Phylogeny, DNA baRcoding

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引用本文

魏亚东, 容万韬, 赵立荣, 王金成, 黄国明, 郭京泽, 孙建华. 五种短体线虫DNA条形码鉴定方法[J]. 华北农学报, 2013, 28(6): 136-139. doi: 10.7668/hbnxb.2013.06.024.

WEI Ya-dong, RONG Wan-tao, ZHAO Li-rong, WANG Jin-cheng, HUANG Guo-ming, GUO Jing-ze, SUN Jian-hua. Identification of 5 PRatylenchus Species Utilizing DNA BaRcoding Method[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 136-139. doi: 10.7668/hbnxb.2013.06.024.

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