摘要： 根据GenBank上已登录的百合斑驳病毒(LMoV)基因组序列设计7对交叉重叠的引物,利用RT-PCR技术对铁炮百合白天堂进行检测,并经克隆测序获得百合斑驳病毒大连分离物(LMoV-DL)基因组全序列。序列分析表明:LMoV基因组由长度为9648bp的线状单链正义RNA组成,包括一个poly(A)尾巴,编码一个由3096个氨基酸组成的分子量为351kDa的多聚蛋白。同时多聚蛋白系统发育分析说明LMoV隶属于Potyvirus属一个较大的分支,在进化关系上与BYMV和ClYVV最近。
关键词:
百合斑驳病毒,
RT-PCR,
基因序列,
系统发育分析
Abstract: According to Lily mottle virus (LMoV) genome sequence logged in the GenBank,we seven pairs of overlapping primers,and use RT-PCR technique to detect Lilium Longiflorum White Heaven,then obtain the whole-genome sequences of Lily mottle virus of Dalian isolates (LMoV-DL) by cloning and sequencing. Through the sequence analysis indicates that: LMoV genome is the linear positive-sense single-stranded RNA which is constituted with the length of 9648 by bp comprising a poly (A) tail and encoding a molecular weight of 351 kDa poly-protein which is composed of 3096 amino acids. Poly-protein phylogeny analysis that the LMoV is a large branch of Potyvirus and close to BYMV and C1YVV on the evolutionary relationship.
Key words:
Lily mottle virus,
RT-PCR,
Gene sequencing,
Phylogenetic analysis
中图分类号:
徐品三, 孙冰轮, 姜旭, 李焕改. 百合斑驳病毒大连分离物基因组全序列测定及其分析[J]. 华北农学报, 2013, 28(S1): 12-15. doi: 10.7668/hbnxb.2013.S1.003.
XU Pin-san, SUN Bing-lun, JIANG Xu, LI Huan-gai. Lily Mottle Virus Genome of Dalian Isolates Sequencing and Its Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(S1): 12-15. doi: 10.7668/hbnxb.2013.S1.003.