基于小RNA技术的天津地区番茄花叶病毒分子检测与基因组部分序列分析

doi:10.7668/hbnxb.20192006

摘要/Abstract

摘要： 为了明确引起天津地区番茄果实褐化的病因，采用小RNA深度测序技术和RT-PCR技术对采自天津的番茄病样进行鉴定分析。结果表明，引起天津地区番茄果实变褐的病原为番茄花叶病毒。进一步对该病毒进行了全基因组测序，并对该病毒的外壳蛋白（CP）、运动蛋白（MP）和126蛋白进行了序列分析。进化树分析结果表明，天津分离物的3个蛋白基因均与美国分离物USA_99-1）的进化关系最近。相似性分析结果表明，CP基因与其他分离物的平均相似性为95.53%。MP基因与其他基因分离物的平均相似性为95.21%。126蛋白基因与其他分离物的平均相似性为92.72%。对这3个基因编码的蛋白进行分析，结果表明，不同分离物之间的平均相似性均在99.0%及以上。初步表明，在天津发生的番茄花叶病毒可能是由于贸易活动由国外传入天津市。

关键词: 番茄, 番茄花叶病毒, 小RNA深度测序, 分子检测, 基因序列分析

Abstract: In order to clarify the cause of tomato fruit browning in Tianjin, deep sequencing of small RNA and RT-PCR were used.The results showed that this symptom was caused by Tomato mosaic virus (ToMV). The sequences of CP(Coat Protein, CP), MP(Movement Protein, MP), and 126 proteins of the virus were further analyzed. Phylogenetic analysis showed that the three genes of the Tianjin isolate(ToMV-TJ) were the closest to the American isolate(USA_99-1).The similarity analysis showed that the average similarity of ToMV-TJ CP gene to other CP gene isolates was 95.53%.The average similarity of MP gene to other MP gene isolates was 95.21%.The average similarity of 126 protein gene to other 126 protein gene isolates was 92.72%.The proteins encoded by these three genes were analyzed. The results showed that the average similarity was above 99.0%. It is initially shown that ToMV-TJ isolate was imported through international trade.

Key words: Tomato, Tomato mosaic virus, Small RNA deep sequencing, Molecular detection, Gene sequence analysis

中图分类号:

S436.412.1+1

金凤媚, 薛俊, 孙海波, 郝志愚. 基于小RNA技术的天津地区番茄花叶病毒分子检测与基因组部分序列分析[J]. 华北农学报, 2021, 36(5): 176-183. doi: 10.7668/hbnxb.20192006.

JIN Fengmei, XUE Jun, SUN Haibo, HAO Zhiyu. Molecular Detection and Partial Genomic Sequence Analysis of Tomato mosaic virus in Tianjin Based on Small RNA Sequencing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 176-183. doi: 10.7668/hbnxb.20192006.

http://www.hbnxb.net/CN/Y2021/V36/I5/176

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