我国芸薹根肿菌遗传类群分化研究

doi:10.7668/hbnxb.2015.S1.056

摘要/Abstract

摘要： 为了明确芸薹根肿菌种群分化与寄主、地理来源之间的关系,以采自我国11个省份,12个寄主上的43份芸薹根肿菌为研究对象,根据肌动蛋白(Actin)和多聚泛素蛋白(Polyubiquitin)这2个位点基因序列进行系统进化分析.结果表明,我国芸薹根肿菌的遗传变异与菌株的地理来源密切相关,可划分为2个遗传类群,其中基于Actin的遗传类群Ⅰ中菌株主要采自北京、四川、山东、河南、黑龙江、上海、陕西、海南、云南、西藏黄河中下游和南部地区,遗传类群Ⅱ中菌株主要采自辽宁、吉林、湖北、云南、西藏东北地区;基于Polyubiquitin的遗传类群Ⅰ中菌株主要采自辽宁、山东、陕西、吉林、河南、海南、云南、西藏华北地区,而遗传类群Ⅱ中菌株主要采自四川、湖北、北京、上海、黑龙江、云南、西藏华南地区.云南、西藏采集菌株在2个遗传类群均有分布.此外,同一寄主的菌株分布于各遗传类群,说明芸薹根肿菌的遗传多样性与寄主来源无关.

关键词: 遗传分化, 芸薹根肿菌, 根肿病, 基因系统发育分析

Abstract: In order to develop a method for genetic differentiation of Plasmodiophora brassicae isolates with host and geographical origin among Chinese isolates,this study analyzed genetic variations of 43 Chinese isolates of P.brassicae from 12 hosts and 11 Provinces.Gene phylogenetic analysis of the isolates was based on actin and polyubiquitin genes,and results showed two divergent clusters Ⅰ and Ⅱ.Based on actin genes,the strains of cluster Ⅰ were collected mainly from middle and lower streams of the Yellow River and South China Beijing,Sichuan,Shandong,Henan,Heilongjiang,Shanghai,Shanxi,Hainan,Yunnan and Xizang Provinces,whereas strains of cluster Ⅱ were collected mainly from Northeast China Liaoning,Jilin,Hubei,Yunnan and Xizang Provinces.Based on polyubiquitin genes,the strains of cluster Ⅰ and cluster Ⅱ were collected from North Liaoning,Shandong,Shanxi,Jilin,Henan,Hainan,Yunnan and Xizang Provinces,and cluster Ⅱ were collected from Sichuan,Hubei,Beijing,Shanghai,Heilongjiang,Yunnan and Xizang Provinces South China,respectively.Besides,isolates from Yunnan and Xizang Provinces were distributed amongst two clusters.Furthermore,strains from the same host were distributed in different genetic groups,which suggested that genetic diversity of isolates of P.brassicae had nothing to do with host species.

Key words: Gene differentiation, Plasmodiophora brassicae, Clubroot, Gene phylogenetic analysis

中图分类号:

S436.3

柴阿丽, 朱发娣, 王惟萍, 石延霞, 谢学文, 李宝聚. 我国芸薹根肿菌遗传类群分化研究[J]. 华北农学报, 2015, 30(S1): 309-315. doi: 10.7668/hbnxb.2015.S1.056.

CHAI A-li, ZHU Fa-di, WANG Wei-ping, SHI Yan-xia, XIE Xue-wen, LI Bao-ju. Genetic Differentiation among Isolates of Plasmodiophora brassicae (Clubroot) from China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 309-315. doi: 10.7668/hbnxb.2015.S1.056.

http://www.hbnxb.net/CN/Y2015/V30/IS1/309

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