摘要： 本试验利用21个RAPD随机引物对50个西瓜枯萎病菌系进行了RAPD扩增，研究了病菌遗传多样性。结果表明，对供试菌系扩增出134条带，其中多态性带113条，占总带数的84.3%。菌系间的遗传相似系数变化在0.45～0.93之间，多数菌系间的同源程度较低。基于RAPD标记聚类分析表明，50个菌系划分为6个RAPD群(RAPD groups，简称RGs)，即 RGⅠ，RGⅡ，RGⅢ，RGⅣ，RGⅤ和RGⅥ。其中RGⅠ和RGⅡ各含有一个菌系，分别来自秦皇岛和新疆；RGⅢ和RGⅤ包括2个菌系，分别来自秦皇岛、承德和廊坊、沧州；RGⅣ包括唐山、石家庄、邯郸和新疆菌系；其余的菌系属于RGⅥ。分类结果进一步说明西瓜枯萎病菌间存在着较大的遗传分化。

关键词: 西瓜, 枯萎病菌, 遗传分化, RAPD

Abstract: Fifty isolates of Fusarium oxysporum f. sp. niveum were RAPD analyzed by using 21 random primers. The result showed that 134 RAPD bands were amplified, among them, 113 (84.3%) bands were polymorphic. Genetic similarity coefficients within the isolates were among 0.45～0.93, there existed a low degree of homogeneity between most of them. When all of polymorphic bands were analyzed by cluster analysis, 50 isolates were classified into 6 groups: Both RG Ⅰ and RG Ⅱ included 1 isolate, respectively, from Qinhuangdao and Xinjiang; RG Ⅲ and RG Ⅴ existed 2 isolates, respectively, from Qinghuangdao, Chengde and Langfang, Cangzhou; RG Ⅵ, It concluded 5 isolates which came from Tangshan, Shijiazhuang, Handan and Xinjiang areas; the others isolates belonged to RG Ⅵ. The result further demonstrated that there existed greater genetic differentiation among watermelon wilt isolates.

Key words: Watermelon, Fusarium oxysporum f. sp. niveum, Genetic different iation, RAPD

中图分类号: 

• Q536.429