一株来源于茼蒿根际的西瓜枯萎病拮抗菌的鉴定及特性研究

doi:10.7668/hbnxb.2016.S1.076

华北农学报 ›› 2016, Vol. 31 ›› Issue (S1): 447-452. doi: 10.7668/hbnxb.2016.S1.076

所属专题： 西瓜；

• 资源环境·植物保护 • 上一篇下一篇

一株来源于茼蒿根际的西瓜枯萎病拮抗菌的鉴定及特性研究

康金磊¹, 韩旭¹, 张仕琦¹, 王丙庆¹, 杨文杰^1,2

1. 华中农业大学园艺林学学院, 湖北武汉 430070;
2. 园艺植物生物学教育部重点实验室, 湖北武汉 430070

收稿日期:2016-07-25 出版日期:2016-12-28
通讯作者: 杨文杰(1974-),男,河北承德人,讲师,博士,主要从事蔬菜作物栽培生理生态研究。
作者简介:康金磊(1994-),男,内蒙古包头人,主要从事蔬菜栽培研究。
基金资助:
中央高校基本科研业务费专项资金资助项目（2013PY092）；华中农业大学大学生科技创新基金（SRF）项目

Screening,Identification and Activity Analysis of Antagonistic Bacteria Against Fusarium Wilt of Watermelon from Chrysanthemum coronarium rhizosphere

KANG Jinlei¹, HAN Xu¹, ZHANG Shiqi¹, WANG Bingqing¹, YANG Wenjie^1,2

1. College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China;
2. Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan 430070, China

Received:2016-07-25 Published:2016-12-28

摘要/Abstract

摘要： 为探讨茼蒿在西瓜枯萎病防治中的作用和效果，并初步探索其作用机制。采用系列稀释法和平板对峙法从水培茼蒿根系悬混液中分离出一株对西瓜枯萎病具有明显拮抗作用的细菌，命名为TK-3。综合菌落形态特征、生理生化特征鉴定和16S rDNA分子鉴定的结果，表明拮抗菌TK-3为解淀粉芽孢杆菌。菌株TK-3中拮抗相关代谢物和生物学性能的研究结果表明：菌株TK-3能够产生吲哚乙酸（IAA）、嗜铁素、NH₃和蛋白酶，具备一定的解磷能力，无HCN的产生。盆栽试验结果表明：拮抗菌TK-3对西瓜枯萎病的防治效果达到48.7%。根据以上结果，TK-3菌株是一株极具开发潜力的生防菌株。

关键词: 拮抗细菌, 西瓜枯萎病, 16S rDNA, 抑菌活性, 解淀粉芽孢杆菌

Abstract: To investigate the roles and effects of Chrysanthemum coronarium in disease controlling on watermelon wilt induced by Fusarium oxysporum f.sp.Niveum (FON).An antagonistic bacterium was isolated from root exudate of Chrysanthemum coronarium rhizosphere.This antagonistic bacterium exhibited significantly ability to watermelon wilt pathogen Fusarium oxysporum f. sp. Niveum (FON) based on the plate confrontation experiment.The TK-3 bacterial strains could antagonize Fusarium oxysporum f. sp. Niveum (FON).In a pot-test, Fusarium wilt of watermelon were reduced by TK-3 strain treated in watermelon were 48.7% of control in 30 days.The results of morphological,physiological and biochemical characterization,and 16S rDNA-based molecular identification showed that the isolated antagonistic bacterium was Bacillus amylolique-faciens.Production of antibacterial metabolites experiments showed strain TK-3 had the ability of IAA production,siderophore production,NH₃ production,proteolytic activity and phosphate solubilization,but could not produce HCN.Results indicated that strain TK-3 had a great potential on bio-control application.

Key words: Antagonistic bacteria, Fusarium wilt of watermelon, 16S rDNA identification, Antifungal metabolites, Bacillus amylolique-faciens

中图分类号:

S436.42
S154

康金磊, 韩旭, 张仕琦, 王丙庆, 杨文杰. 一株来源于茼蒿根际的西瓜枯萎病拮抗菌的鉴定及特性研究[J]. 华北农学报, 2016, 31(S1): 447-452. doi: 10.7668/hbnxb.2016.S1.076.

KANG Jinlei, HAN Xu, ZHANG Shiqi, WANG Bingqing, YANG Wenjie. Screening,Identification and Activity Analysis of Antagonistic Bacteria Against Fusarium Wilt of Watermelon from Chrysanthemum coronarium rhizosphere[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 447-452. doi: 10.7668/hbnxb.2016.S1.076.

http://www.hbnxb.net/CN/Y2016/V31/IS1/447

参考文献

[1] 李怀方,刘凤权,郭小密.园艺植物病理学[M].北京:中国农业大学出版社,2001:246.
[2] 吴洪生,周晓冬,李鹤,等.黄瓜、西瓜枯萎病拮抗细菌的初步分离与鉴定[J].西南农业学报,2013,26(3):1019-1025.
[3] 孙正祥,王丰,黄玲,等.西瓜枯萎病生防菌XG-1的筛选、鉴定及其抑菌作用[J].江西农业大学学报,2013,35(2):324-328.
[4] 岳菊,刘邮洲,张荣胜,等.西瓜枯萎菌拮抗细菌的筛选,鉴定及防效测定[J].中国生物防治学报,2011,27(3):428-432.
[5] Ling N,Huang Q W,Guo S W,et al. Paenibacillus polymyxa SQR-21 systemically affects root exudates of watermelon to decrease the conidial germination of Fusarium oxysporum f.sp. Niveum[J].Plant and Soil,2011,341(1/2):485-493.
[6] 宋顺华,吴萍,邢宝田,等.多粘类芽胞杆菌WY110对西瓜枯萎病的控制作用[J].植物保护学报,2011,38(6):571-572.
[7] 赵丽明,丁延芹,路晓萌,等.西瓜根际枯萎病拮抗放线菌的筛选及鉴定[J].生物技术通报,2010(5):107-110.
[8] 纪明山,王英姿,程根武,等.西瓜枯萎病拮抗菌株筛选及田间防效试验[J].中国生物防治,2002,18(2):71-74.
[9] 赵国其,林福呈,陈卫良.绿色木霉对西瓜枯萎病苗期的控制作用[J].浙江农业学报,1998,10(4):206-209.
[10] Latz E,Eisenhauer N,Rall B C,et al.Plant diversity improves protection against soil-borne pathogens by fostering antagonistic bacterial communities[J].Journal of Ecology,2012,100(3):597-604.
[11] 曹云,马艳.间套作防治作物土传枯萎病的研究进展[J].土壤,2015,47(3):466-473.
[12] 姜丽.茼蒿对西瓜化感作用的初步研究[D].合肥:安徽农业大学,2007:33-34.
[13] 张宁,张如,吴萍,等.根系分泌物在西瓜/旱作水稻间作减轻西瓜枯萎病中的响应[J].土壤学报,2014,51(3):585-593.
[14] 任丽轩.旱作水稻/西瓜间作抑制西瓜枯萎病的生理机制[D].南京:南京农业大学,2012.
[15] 杨智仙,汤利,郑毅,等.不同品种小麦与蚕豆间作对蚕豆枯萎病发生、根系分泌物和根际微生物群落功能多样性的影响[J].植物营养与肥料学报,2014,20(3):570-579.
[16] 董艳,董坤,郑毅,等.不同品种小麦与蚕豆间作对蚕豆枯萎病的防治及其机理[J].应用生态学报,2014,25(7):1979-1987.
[17] 沈萍,范秀荣,李广武.微生物学实验[M].北京:人民教育出版社,1980:69-82.
[18] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[19] Bric J M,Bostock R M,Silverstone S E.Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane[J].Applied and Environmental Microbiology,1991,57(2):535-538.
[20] Schwyn B,Neilands J B.Universal chemical assay for the detection and determination of siderophores[J].Analytical Biochemistry,1987,160(1):47-56.
[21] Bakker A W,Schippers B.Microbial cyanide production in therhizosphere in relation to potato yield reduction and Pseudomonas spp.mediated plant growth stimulation[J].Soil Biology and Biochemistry,1987,19(4):451-457.
[22] Nautiyal C S.An efficient microbiological growth medium for screening phosphate solubilizing microorganisms[J].FEMS Microbiology Letters,1999,170(1):265-270.
[23] Kumar R S,Ayyadurai N,Pandiaraja P,et al.Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits[J].Journal of Applied Microbiology,2005,98(1):145-154.
[24] Cappuccino J G,Sherman N.Microbiology-A Laboratory Manual[M].1st ed.California:The Benjamin/Cummings Publishing Co,1992:105-300.
[25] Alvindia D G.Enhancing the bioefficacy of Bacillus amyloliquefaciens DGA14 with inorganic salts for the control of banana crown rot[J].Crop Protection,2013,51(3):1-6.
[26] Yuan S,Wang L,Wu K,et al.Evaluation of Bacillus-fortilied organic fertilizer for controlling tobacco bacterial wilt in greenhouse and field experiments[J].Applied Soil Ecology,2014,75:86-94.
[27] Alvarez F,Castro M,Príncipe A,et al.The plant-associated Bacillus amyloliquefaciens strains MEP218 and ARP23 capable of producing the cyclic lipopeptides iturin or surfactin and fengycin are effective in biocontrol of sclerotinia stem rot disease[J].Journal of Applied Microbiology,2012,112(1):159-174.
[28] Chen X H,Koumoutsi A,Scholz R,et al.Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens[J].Journal of Biotechnology,2009,140(1/2):27-37.

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一株来源于茼蒿根际的西瓜枯萎病拮抗菌的鉴定及特性研究

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