内生细菌S258产挥发性有机物的抑菌促生长活性及其组分分析

doi:10.7668/hbnxb.20192376

摘要/Abstract

摘要： 为了更好地利用分离自棉花根部的内生拮抗细菌S258进行生物防治，特别是利用其产生的挥发性有机物进行植物病害的防治，以棉花黄萎菌和西瓜枯萎菌作为靶标病原真菌，以拟南芥作为指示植物，通过二分格培养皿对峙培养法测定其产生的挥发性有机物对病原真菌的抑菌活性，以及对植物的促生长活性。结果表明，内生细菌S258产生的挥发性有机物对病原真菌的生长具有抑制作用，并且能够促进植物的生长。与阴性对照大肠杆菌DH5α相比，对棉花黄萎菌和西瓜枯萎菌菌丝生长的抑制率分别达到80.9%，10.1%。与不加细菌的空白对照相比，对拟南芥的生长具有很强的促进作用，使拟南芥单株鲜质量达到空白对照的5.3倍。用顶空固相微萃取法收集内生细菌S258产生的挥发性有机物，再用气质联用的方法，分析其挥发性有机物的组成，经NIST和WILIY质谱数据库自动检索，鉴定得到其挥发性有机物包含30种单体化合物，分别属于烷烃、烯烃、醇、酮、醚、醛、酯及杂环类。根据总离子流图的峰面积判断，主要气体成分为二甲基二硫醚和吲哚。综上所述，内生细菌S258能够产生具有抑菌促生长活性的挥发性有机物，是开发为生防产品的重要微生物资源，其挥发性有机物也具有较高的研究和应用价值。

关键词: 内生细菌, 挥发性有机物, 抑菌活性, 促生长活性

Abstract: In order to further utilization of the endophytic antagonistic bacterium S258 isolated from cotton root for biological control, especially to use the volatile organic compounds(VOCs) produced by the strain to control plant disease, Verticillium dahliae and Fusarium oxysporum f. sp. niveum were used as the target pathogenic fungi, and Arabidopsis thaliana was used as the indicator plant, the antifungal activity of the VOCs from the strain against pathogenic fungi and the growth promoting activity against plants were determined by the dual culture on two-section of a Petri dish. The results showed that the VOCs from the endophytic bacterium S258 could inhibit the mycelial growth of pathogenic fungi and at the same time promoted the plant growth. Compared with the negative control E. coli DH5α, the growth inhibition rate of the VOCs against Verticillium dahliae and Fusarium oxysporum f. sp. niveum were 80.9% and 10.1%, respectively. Compared with the blank control without bacteria, the fresh weight per plant of Arabidopsis thaliana treated by the VOCs was 5.3 times that of the blank control. The VOCs from the endophytic bacterium S258 were collected by headspace solid-phase microextraction. Then the compositions of VOCs were analyzed by GC-MS. The VOCs were identified by NIST and WILIY mass spectrometry databases as 30 monomer compounds, belonging to alkanes, alkenes, alcohols, ketones, ethers, aldehydes, esters and heterocycles. According to the peak area of total ion current graph, dimethyl disulfide and indole were most abundant among these compounds. In conclusion, the endophytic bacteria S258 can produce volatile organic compounds with antifungal and growth-promoting activity, which is an important microbial resource for the development of biocontrol products, and its volatile organic compounds also have high research and application value.

Key words: Endophytic bacteria, Volatile organic compounds, Antifungal activity, Growth-promoting activity

中图分类号:

S432.1

罗晓宇, 单晨阳, 贾召召, 张昕, 林玲. 内生细菌S258产挥发性有机物的抑菌促生长活性及其组分分析[J]. 华北农学报, 2021, 36(5): 198-203. doi: 10.7668/hbnxb.20192376.

LUO Xiaoyu, SHAN Chenyang, JIA Zhaozhao, ZHANG Xin, LIN Ling. The Antifungal and Plant Growth-Promoting Effect and Components Analysis of the Volatile Organic Compounds from an Endophytic Bacterium S258[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 198-203. doi: 10.7668/hbnxb.20192376.

http://www.hbnxb.net/CN/Y2021/V36/I5/198

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