摘要： 通过热水提取法提取灵芝多糖,得到灵芝粗多糖,并以3种植物病原菌(胡萝卜欧氏菌、指状青霉、灰葡萄孢)和5种食品有害菌(蜡状芽孢杆菌、枯草芽孢杆菌、大肠杆菌、黑曲霉和黑根霉)为供试菌,采用纸片琼脂法来测定灵芝粗多糖对供试菌的抑菌强度。结果表明,灵芝粗多糖对植物病原菌中的胡萝卜欧氏菌有较强的抑制作用;对指状青霉菌有一定的抑制作用,但抑制作用较弱;对灰葡萄孢几乎没有抑制作用;对食品有害菌中的枯草芽孢杆和蜡状芽孢杆菌有很强的抑制作用;对大肠杆菌和黑曲霉抑制作用相对较弱;对黑根霉几乎没有抑制作用。总体来看,灵芝多糖对细菌的抑菌活性强于真菌。

关键词: 灵芝多糖, 抑菌活性, 抑制作用

Abstract: The polysaccharides from Ganoderma lucidum by boiling water method was extracted. These crude extracts were tested for antimicrobial activitiy to three kinds of plant pathogens (Erwinia carotovora, Penicillium digitatum, Botrytis cinerea) and five kinds of food farmful bacteria(Bacillus cereus, Bacillus subtilis, Escherichiacoli, Aspergillus niger and Rhizopus nigricans) with the agar diffusion method. The results showed that the polysaccharides liquid had a powerful inhibiting effect on Erwinia carotovora, a weak inhibiting effect on Penicillium digitatum and nearly non- in?? hibiting effect on Botrytis cinerea for the plant pathogens. For the food harmful bacteria, the polysaccharides liquid had a strong inhibiting effect on Bacillus subtilis and Bacillus cereus, a weak inhibiting effect on Escherichia coli and As pergillus niger, nearly non??inhibiting effect on Rhizopus nigricans. In a word, the antimicrobial activity of polysaccha?? rides from Ganoderma lucidum was higher on bacteria than that of on fungi.

Key words: Ganoderma lucidum polysaccharides, Antimicrobial activity, Inhibiting effect

中图分类号: 

• R285